Effect of Sleep Restriction on Skeletal Muscle in Post-menopausal women
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
PROVIDER: GSE309217 | GEO | 2026/05/15
REPOSITORIES: GEO
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Project description:Both sleep loss and exercise regulate gene expression in skeletal muscle, yet little is known about how the interaction of these stressors affects the transcriptome. The aim of this study was to investigate the effect of nine nights of sleep restriction, with repeated resistance exercise (REx), on the skeletal muscle transcriptome of young, trained females.
2025-04-12 | GSE263838 | GEO
Project description:Introduction: Sleep deprivation is associated with increased cardiovascular risk, which is more pronounced in women than men; however, causal evidence is lacking and the underlying mechanisms are unclear. We used randomized crossover design of prolonged sleep deprivation that mimics “life-like conditions” and endothelial cells (ECs) harvested from healthy women to investigate directly whether sleep deprivation impairs endothelial function and to identify underlying mechanisms. Methods: Healthy women with normal habitual sleep (7-9 h/day) were randomly allocated to 6-weeks of adequate sleep (7-9 h/day) or sleep deprivation (1.5 h less than habitual sleep) in a crossover design. Sleep duration was monitored objectively by actigraphy. EC harvesting and brachial artery flow-mediated dilation (FMD) were performed at baseline and the end of adequate sleep and sleep deprivation period. Results: Twenty-eight healthy women (mean [SE] age 35±13 years; BMI 25±3 kg/m2) participated. Compared with adequate sleep, sleep deprivation reduced FMD (mean±SE 8.65±0.48 vs. 7.35±0.39, p=0.02) and increased EC inflammation (nuclear factor-κB nuclear fluorescence area mean±SE 1.36±0.24 vs. 2.04±0.38 μm2, p=0.03 and mRNA expression of vascular cell adhesion molecule-1 mean±SE 1.00±0.19 vs. 2.31±0.72, p=0.04) compared with adequate sleep. Sleep deprivation increased EC oxidative stress by 67% compared with adequate sleep (redox sensitive fluorogenic probe fluorescence intensity, p=0.002) without upregulating antioxidant response. Using RNA-seq and a predicted protein-protein interaction algorithm, we identified reduced expression of serum response factor, a transcription factor that primes cortical response to sleep deprivation, and its endothelial target Defective in Cullin Neddylation-1 Domain Containing 3 as novel mediators of impaired nuclear factor (erythroid-derived 2)-like 2-mediated antioxidant responses in ECs in sleep deprivation. Conclusion: Sleep deprivation impairs clearance of endothelial oxidant stress accumulated during wakefulness leading to endothelial dysfunction and potentially increased cardiovascular risk in women. Trial Registration Number: ClinicalTrials.gov NCT02835261
2024-04-05 | GSE171831 | GEO
Project description:<p>As sleep loss leads to accidents and impaired safety, a direct metabolic marker would be beneficial for forensic interpretation. </p><p>In a sufficiently powered, randomized, controlled, crossover trial under realistic conditions, we examined the salivary metabolome of 20 young men (habitual sleep duration 7–9 h) following three interventions: one night of total sleep deprivation, four consecutive nights of sleep restriction to 6 h, and control (8 h of sleep). Oral fluid specimens were repeatedly collected and analyzed using liquid chromatography coupled to mass spectrometry. Logistic regression models were trained to classify unseen samples without reference samples from the same individual. </p><p>Acute sleep deprivation exhibited a unique metabolic fingerprint that could be detected precisely (F0.5 = 0.90) when using only 12 molecular features. This fingerprint was more pronounced in samples collected in the morning/midday hours. Nevertheless, at all time points, the overall correct predictions by far outweighed the incorrect ones. Four nights of sleep restriction did not lead to exploitable metabolic changes. </p><p>This study presents a metabolic fingerprint of acute sleep deprivation in oral fluid under realistic conditions and explores practical implications and limitations of its machine learning-aided classification. Metabolomics-based, reference-free sleep loss detection holds potential for applications in forensic, clinical, and occupational contexts.</p>
2026-05-21 | MTBLS11413 | MetaboLights
Project description:Adequate sleep (AS) is essential for maintaining many behavioral and physiological functions; however, a third of adults in the United States sleep less than the recommended 7-9 h/night. The circadian rhythm controls tissue homeostasis, in part, through adult stem cell functions. However, little is known about the mechanistic interactions between insufficient sleep and stem cell functions. Using plasma collected from healthy women with adequate habitual sleep duration (7-9 h/night) who were randomized in a crossover design to a 6-week objectively monitored AS or mildly restricted sleep (RS; 1.5 h less than their AS), we identified metabolites and proteins differentially enriched after RS compared to AS. RS induced a stress-like state dominated by the ATF6 interactome, heat shock, and ubiquitination proteins. Joint network pathway analysis highlights a strong link between RS and neuronal development phenotypes. Using neural stem cells (NSCs) differentiated from human embryonic stem cells, we found treatment with metabolic candidates enriched in restricted sleep led to aberrant G1 phase of the cell cycle and defective differentiation of NSCs into neurons, astrocytes, and oligodendrocytes. Thus, restricted sleep that mimics “life-like” conditions can influence the daily dynamics of NSC divisions and differentiation, shedding light on how sleep can shape adult stem cell functions in neural development.
2025-04-07 | GSE254828 | GEO
Project description:We investigated the genomic and physiological impact of acute sleep loss in peripheral tissues, by obtaining adipose tissue and skeletal muscle after one night of sleep loss and after one full night of sleep. Processed data (count table) only. Raw data will be submitted to EGA.
2018-06-30 | E-MTAB-6903 | biostudies-arrayexpress
Project description:We investigated the genomic and physiological impact of acute sleep loss in peripheral tissues, by obtaining adipose tissue and skeletal muscle after one night of sleep loss and after one full night of sleep. Processed data (M-values for probes not overlapping SNPs) only. Raw data will be submitted to EGA.
2018-06-30 | E-MTAB-6908 | biostudies-arrayexpress
Project description:Short sleep duration is associated with adverse metabolic, cardiovascular, and inflammatory effects. Co-twin study methodologies account for familial (e.g., genetics and shared environmental) confounding, allowing assessment of subtle environmental effects, such as the effect of short habitual sleep duration on gene expression. Therefore, we sought to investigate gene expression in monozygotic twins discordant for actigraphically phenotyped habitual sleep duration. Eleven healthy monozygotic twin pairs (82% female; mean age 42.7 years; SD=18.1), selected based on subjective sleep duration discordance, were objectively phenotyped for habitual sleep duration with two-weeks of wrist actigraphy. Peripheral blood leukocyte (PBL) RNA from fasting blood samples was obtained on the final day of actigraphic measurement and hybridized to Illumina humanHT-12 microarrays. Differential gene expression was determined between paired samples and mapped to functional categories using Gene Ontology. Next, a more comprehensive gene set enrichment analysis was performed based on the entire PBL transcriptome. The mean 24 hour sleep duration of the total sample was 439.2 minutes (SD=46.8 minutes; range 325.4 to 521.6 minutes). Mean within-pair sleep duration difference per 24 hours was 64.4 minutes (SD=21.2; range 45.9 to 114.6 minutes). The twin cohort displayed distinctive pathway enrichment based on sleep duration differences. Short sleep was associated with up-regulation of genes involved in transcription, ribosome, translation and oxidative phosphorylation. Unexpectedly, genes down-regulated in short sleep twins were highly enriched in immuno-inflammatory pathways such interleukin signaling and leukocyte activation, as well as developmental programs, coagulation cascade, and cell adhesion. Objectively assessed habitual sleep duration in monozygotic twin pairs appears to be associated with distinct patterns of differential gene expression and pathway enrichment. By accounting for familial confounding and measuring real life sleep duration, our study shows the transcriptomic effects of short sleep on dysregulated immune response and provides a potential link between sleep deprivation and adverse metabolic, cardiovascular and inflammatory outcomes.
2016-09-09 | GSE80612 | GEO
Project description:How sleep influences the transcriptional programing of blood leukocytes in humans remains incompletely undertstood. Volunteers with adequate sleep participated in a 6 week cross-over design trial. During the habitual sleep (HS) phase, participants did not alter their sleep and slept adequately. During the sleep restriction phase (SR), participants restricted their sleep by ∼1.5 hours per night. PBMCs from 8 individuals (4 HS and 4 SR) were collected subjected to scRNAseq.
2024-10-30 | GSE275071 | GEO
Project description:Healthy human adults were recruited to a sleep lab at Washington State University and remained there 7 consecutive days. Six received a well-rested Control condition of 10 h Time-In-Bed (TIB) nightly. Another 11 subjects underwent Sleep Deprivation. They had a Baseline with 10 h TIB, followed by an Experimental phase of 62 h continued wakefulness, and finally two nights of 10 h TIB before dismissal. Blood draws on one Baseline day, one Experimental day, and one Recovery day were used for gene expression microarrays.
2018-04-10 | GSE98564 | GEO
Project description:Healthy human adults were recruited to a sleep lab at Washington State University and remained there 7 consecutive days. Six received a well-rested Control condition of 10 h Time-In-Bed (TIB) nightly. Another 11 subjects underwent Sleep Deprivation. They had a Baseline with 10 h TIB, followed by an Experimental phase of 62 h continued wakefulness, and finally two nights of 10 h TIB before dismissal. Blood draws on one Baseline day, one Experimental day, and one Recovery day were used for gene expression microarrays.
2018-04-10 | GSE98565 | GEO