Transcriptome profiling of developing inflorescences reveals SiMADS1 as a key regulator of floral development in foxtail millet [DAP-seq]

ABSTRACT: Inflorescence development of foxtail millet (Setaria italica L.) is important for its yield and quality. The molecular basis of foxtail millet inflorescence development is poorly understood. In this study, we analyzed the transcriptome landscape of three developing inflorescence stages in foxtail millet and compared with two-week seedlings. The transcriptome revealed the expression model of genes that may be engagement in inflorescence development, such as kinase, transporter, E3 ubiquitin ligase, reactive oxygen species (ROS) related proteins, MADS-box family, shoot apical meristem pathway related proteins, cell cycle associated proteins, ribosomal proteins and etc. The SiMADS1 knockout line exhibits a phenotype of defective floral organs, resulting in greatly reduced yield. We further analyzed the transcriptomes of simads1 at the green anther and panicle stages. By integrating transcriptomes data with DAP-seq analysis, we identified multiple downstream regulatory genes of SiMADS1. This study provides new genetic resources for further analysis of the molecular mechanism of inflorescence development in foxtail millet.

ORGANISM(S): Setaria italica

PROVIDER: GSE309337 | GEO | 2026/09/01

REPOSITORIES: GEO