Identification of ALKBH5-interacting RNAs in lung cancer cells by RNA immunoprecipitation sequencing (RIP-Seq)
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ABSTRACT: This experiment was performed to identify the RNAs, particularly lncRNAs, that bind to the m6A demethylase ALKBH5 under nutrient stress. Lysates from 95D cells cultured under glucose- or glutamine-deficient conditions were subjected to immunoprecipitation using an antibody against ALKBH5. Co-precipitated RNAs were isolated, used to construct sequencing libraries, and analyzed to identify enriched transcripts.
ORGANISM(S): Homo sapiens
PROVIDER: GSE309436 | GEO | 2025/10/27
REPOSITORIES: GEO
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