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Effects of Moringa oleifera extracellular vesicles on human microRNAs involved in the HeLa cell tumorigenesis

ABSTRACT: The effects of extracellular vesicles from Moringa oleifera on the modulation of tumour associated human-microRNAs profile in cervical cancer-derived HeLa cells were investigated. Plant extracellular vesicles have been shown to carry a variety of bioactive compounds, such as proteins, lipids, phytochemicals (e.g., phenols and flavonoids), and RNAs, including nucleic acids (e.g., miRs), mediating the transfer of information to recipient cells and thus modulating their function and gene expression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE309748 | GEO | 2025/10/02

REPOSITORIES: GEO

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Project description:MicroRNAs (miRNAs) are endogenous, noncoding, smallRNAs that regulate gene expression at the post-transcriptional level during plant development, growth and seed germination. Among all medicinal plants, Moringa oleifera is one of the most useful trees for which, despite its diffusion, no information about its miRNAs and their respective target genes is available. In this research, we report results obtained from a high-throughput sequencing analysis performed with the Illumina platform. A total of 31,290,964 raw reads were produced from M. oleifera seed smallRNA library. First, we found 99 conserved miRNAs and 43 novel ones that we partially validated by qRT- PCR. Second, by comparing their expression abundances with those of other common plants, we identified 20 conserved M. oleifera miRNAs. For both these results an in silico analysis allowed us to predict some of their targets which in turn allowed us to link them to a wide range of physiological processes. Based on qRT-PCR expression analyses, we reported the expression profile of some selected conserved miRNAs in different M. oleifera tissues (roots, stems and leafs). We compared the most conserved miRNAs found in Moringa with those of other edible plants, such as Olea europaea and Brassica rapa. Furthermore, by taking advantage of a recently developed web- application based on an algorithm that compares plants and mammalian miRNAs, we identify a few possible plant miRNAs with functional homologies with mammalian ones. We used the 20 most abundant M. oleifera miRNAs to conduct a preliminary analysis to investigate potential cross-kingdom gene regulation. To our knowledge, this is the first report of M. oleifera miRNAs that uses high-throughput sequencing analysis. Our analysis increases the amount of information about plant miRNAs currently available and it can help us understanding the molecular mechanism of this medical plant. microRNA profile of M. oleifera seed, germinated on paper soaked in sterile water.

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Effects of Moringa oleifera extracellular vesicles on human microRNAs involved in the HeLa cell tumorigenesis

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