Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of Recombinant ApoE on Gene Expression During Osteoblast Differentiation of Mouse Bone Marrow Stromal Cells (BMSCs)

ABSTRACT: Advanced age impairs bone fracture healing; however, the underlying mechanisms remain unclear. We found that circulating levels of apolipoprotein E (ApoE) increase with age and contribute to impaired fracture healing. In vitro, ApoE treatment of aged bone marrow stromal cells (BMSCs) inhibited osteoblast differentiation. To investigate the mechanism by which ApoE inhibits osteoblast differentiation, we treated primary BMSCs isolated from the femurs and tibiae of 24-month-old C57BL6/J mice with recombinant ApoE. For osteoblast differentiation, BMSCs were cultured in osteogenic medium. Recombinant ApoE (rApoE) was added at a concentration of 100 ng/mL starting two days after induction of differentiation and continued throughout the 10-day differentiation period. BMSCs treated with vehicle or rApoE were harvested on day 10. Total RNA was extracted using TRIzol Reagent (Invitrogen) according to the manufacturer’s protocol for downstream transcriptomic analysis.

ORGANISM(S): Mus musculus

PROVIDER: GSE309814 | GEO | 2025/12/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

miRNA expression in BMSCs

Project description:We performed microarray analysis to determine the expression profiles of miRNAs during osteoblast differentiation of BMSCs

2019-03-11 | GSE115197 | GEO

circRNA expression in BMSCs

Project description:We performed microarray analysis to determine the expression profiles of circRNAs during osteoblast differentiation of BMSCs

2019-03-11 | GSE115196 | GEO

Single-cell RNA-seq analysis reveals reduction of B cells in the bone marrow promote fracture healing

Project description:Recent studies have identified B cells inhibit bone formation in rheumatoid arthritis by suppressing osteoblast differentiation. However, it remains unknown how B cells participate in the healing of fractures. Here we find that B cells decrease significantly during fracture healing and returned to normal levels after that by single-cell profiling. Furthermore, we found that exosomes derived from B cells inhibited the differentiation of osteoblasts and osteoclasts in vitro. Additionally, injection of these exosomes in mice delayed fracture healing. Thus, maintaining low B cell levels in the bone marrow microenvironment promotes fracture healing.

2021-11-01 | GSE142786 | GEO

Extracellular vesicles from osteogenic differentiated human BMSCs

Project description:Pathological processes like osteoporosis or steroid-induced osteonecrosis of the hip are accompanied by increased bone marrow adipogenesis. Such disorder of adipogenic/osteogenic differentiation, which affects also bone marrow derived mesenchymal stem cells (BMSCs) contributes to bone loss during aging. Therefore, we investigated the effects of extracellular vesicles (EVs) isolated from human (h)BMSCs during different stages of osteogenic differentiation on osteogenic and adipogenic differentiation capacity of naïve hBMSCs.

2022-10-14 | PXD035845 | Pride

Identification of early response genes to low-intensity pulsed ultrasound in bone marrow stromal cells

Project description:Low-intensity pulsed ultrasound (LIPUS) has been applied as a therapeutic adjunct to promote fracture healing. However, the detailed molecular mechanisms by which LIPUS promotes bone fracture healing have not yet been fully elucidated. In the present study, the early response genes elicited by low-intensity pulsed ultrasound (LIPUS) in bone marrow stromal cells (BMSCs) were investigated using GeneChip® oligonucleotide microarrays.

2019-08-19 | GSE135935 | GEO

Conditional Knockout of Fshr in Osteocytes Restores Bone Mass and Accelerates Fracture Healing in Ovariectomized Mice

Project description:Building on our previous observation that osteocytes express FSH receptors (FSHR), we generated Dmp1-CreERT; Fshrfl/fl mice and subjected them to ovariectomy (OVX), followed by weekly tamoxifen administration to achieve osteocyte-specific Fshr deletion. Osteocyte-specific Fshr knockout mice exhibited increased bone mineralization alongside elevated bone resorption. RNA-seq of femoral cortical bone revealed enhanced coupling of bone formation and resorption, which was associated with PI3K/Akt pathway activation. Digital PCR and ELISA confirmed up-regulation of osteoblast- and osteoclast-related genes. In vitro, Fshr deletion abolished FSH-mediated inhibition of the PI3K/Akt pathway and restored osteogenic mineralization of bone-marrow mesenchymal stem cells (BMSCs). In a closed femoral-fracture model, osteocyte-specific Fshr knockout accelerated callus maturation and fracture healing, and three-point bending tests demonstrated improved biomechanical properties. These findings identify osteocytes as direct FSH-responsive cells within bone. Osteocyte-specific Fshr deletion enhances PI3K/Akt signaling, enhancing formation–resorption coupling, and accelerates fracture repair in ovariectomized mice. Our study provides mechanistic insights and potential therapeutic strategies for postmenopausal osteoporosis and fracture management.

2025-12-01 | GSE305679 | GEO

S7, E7 peptide action on BMSCs

Project description:Pro-vascular differentiation and adhesion of BMSCs by S7 and E7 peptides

2024-09-23 | GSE246630 | GEO

Expression data from young and aged BMSCs

Project description:Bone marrow mesenchymal stem cells (BMSCs) differentiate into various mature cell types, including adipocytes and osteoblasts, which is determined by genetic, molecular mediators and local microenvironment. With age, BMSCs become inclined to undergo differentiation into adipocytes rather than osteoblasts, resulting in an increased number of adipocytes and a decreased number of osteoblasts, causing osteoporosis. The dysregulated the gene expression in BMSCs during aging were analyzed. We used microarrays to detail the global programme of gene expression duing aing in BMSCs.

2018-08-15 | GSE116925 | GEO

miRNA as biomarkers for fracture healing

Project description:Fracture non-union is a complex clinical condition affecting many patients worldwide; however, known risk factors alone cannot help in predicting individual risk of progressing towards healing complications. The identification of novel biomarkers in the serum of fracture patients is crucial for early diagnosis and timely patient treatment. This study focused on the identification of microRNA (miRNA) that could correlate to the process of fracture healing. Toward this aim, serum of fracture patients and healthy volunteers was screened by RNA sequencing to identify differentially expressed miRNA at different times after injury. The results were correlated to miRNA that were found to be differentially secreted in the conditioned medium of human bone marrow mesenchymal stromal cells (BMSCs) during in vitro osteogenesis.

2024-06-02 | GSE217879 | GEO

Proteomics analysis of early stages of human osteoblasts osteogenic differentiation in vitro

Project description:Cellular mechanisms and signaling cascades underlying the early stages of osteoblast progenitors differentiation in adult bone are still not well understood. Therefore, we performed shotgun proteomics analysis of primary culture of isolated human osteoblasts from femur of adult donors in control and on the sixth day of osteogenic differentiation in vitro. This is an early timepoint in which we have observed no extracellular matrix mineralization yet. Nevertheless, for protein extraction we used acidic extraction method which is effective for bones and other mineralized tissues. 1785 proteins identified with at least two unique peptides were included in proteomics analysis. We revealed physiological shift associated with a decrease of cells proliferative activity and extracellular matrix secretion and organization. The obtained data can make a significant contribution to understanding processes of osteogenesis induction to enhance fracture healing.

2022-12-25 | PXD033697 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data