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Bulk RNA-seq of the CD and LPD-fed mice injected with vector control or c-Met/ΔN90-β-catenin by SB-HTVI

ABSTRACT: We performed bulk RNA-seq to compare liver tissues from 4 groups of mice: vector control fed with CD, c-Met/β-cat mice fed with CD, vector control fed with LPD, and c- Met/β-cat mice fed with LPD. Differential gene expression and gene set enrichment analyses (GSEA) were performed and identified two clusters that might help explain the differential tumor development in the CD and LPD groups.

ORGANISM(S): Mus musculus

PROVIDER: GSE310077 | GEO | 2025/12/08

REPOSITORIES: GEO

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Project description:The importance of parental diet in relation to eventual offspring health is increasing in prominence due to the increased frequency of reproductive age adults consuming poor diets. Whilst maternal health and offspring outcome have been studied in some detail, the paternal impacts are not as well understood. A father’s poor nutritional status has been shown to have negative consequences on fetal growth and development and ultimately impact the long-term adult health of the offspring. In this study we examined sperm and seminal plasma mediated mechanisms of preimplantation embryo development alterations in response to sub-optimal paternal diets. Male mice were fed a diet to model either under (low protein diet (LPD)) or over (high fat/sugar (Western) diet (WD)) nutrition, LPD or WD supplemented with methyl-donors or a control diet before mating with age-matched control fed females. Male metabolic health was influenced by WD and MD-WD; with significant changes in serum lipids and hepatic 1-carbon metabolites. Sperm RNA sequencing revealed significant changes to mRNA profiles in all groups when compared to CD (LPD: 32, MD-LPD: 17, WD: 53, MD-WD: 35). In vitro embryo development was revealed all sub-optimal diets with and without methyl-donors increased the rate of embryo development. As embryo development can be directed by sperm and seminal plasma mediated mechanism, we also examined the proteomic profile of the seminal plasma, revealing a significant number of changes in all groups compared to control (LPD: 13, MD-LPD: 27, WD: 24, MD-WD: 19). Male diet also altered uterine gene expression examined via qPCR, specifically alterations in Cd14 and Ptgs1 were observed in response to male WD matings. Our current study shows that paternal nutritional status has the potential to influence male metabolic and reproductive factors, which can ultimately alter embryonic development and the post-mating maternal environment. This study highlights potential direct (sperm mediated) and indirect (semen mediated) pathways in which a father's poor diet could shape the long-term health of his offspring.

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Bulk RNA-seq of the CD and LPD-fed mice injected with vector control or c-Met/ΔN90-β-catenin by SB-HTVI

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