Placental Exosomal miR-24-1-5p in LPS-Induced Inflammation
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ABSTRACT: Intra-amniotic infection (IAI) is associated with substantial maternal and neonatal risks, yet the immunoregulatory role of the human placenta remains incompletely understood. We established an in vitro IAI model by co-culturing term human placental explants with lipopolysaccharide (LPS) and monitored temporal changes in inflammatory cytokines. Prolonged LPS exposure induced an early pro-inflammatory phase followed by an anti-inflammatory phase characterized by decreased TNF-α/IL-10 and IL-1β/IL-1Ra ratios. Placenta-derived exosomes (Pd-Exos) were isolated from culture supernatants and subjected to miRNA sequencing, which identified miR-24-1-5p as a key upregulated miRNA in Pd-Exos from LPS-treated explants (1-LPS-Pd-Exos). After uptake by THP-1–derived macrophages, exosomal miR-24-1-5p targeted TNFAIP8, promoted macrophage apoptosis, and suppressed TNF-α and IL-1β expression, thereby contributing to placental endotoxin tolerance.
ORGANISM(S): Homo sapiens
PROVIDER: GSE310377 | GEO | 2025/11/28
REPOSITORIES: GEO
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