Aristaeus chip 1.0: an in situ synthesized oligonucleotide microarray for Ovis aries

ABSTRACT: Here we present a high-density in situ synthesized microarray for Ovis aries, named Aristaeus, designed by means of a pipeline of software instruments that, starting from non-annotated redundant EST sequences, selects oligonucleotides suitable for in situ generation on chip. The chip was tested by comparing the gene expression profiles of two sheep breeds with different phenotype, Sarda and Gentile di Puglia. We carried out microarray experiments on liver and udder tissues from lactating individuals and identified a relevant number of differentially expressed genes, all involved in metabolism pathways. The results are consistent with literature knowledge, while selected differential gene expressions have been confirmed by quantitative real-time polymerase chain reaction analyses.

ORGANISM(S): Ovis aries

PROVIDER: GSE31044 | GEO | 2013/02/05

SECONDARY ACCESSION(S): PRJNA144873

REPOSITORIES: GEO