Dataset Information

Different effects of 3-week disuse on the phenotype and gene expression of the calf and thigh muscles

ABSTRACT: Disuse, like several other pathological conditions, has specific effects on various skeletal muscles. The mechanisms underlying these responses remain unclear. Three-week bed rest resulted in a greater decrease in lean mass, endurance, and more pronounced changes in the proteome and transcriptome of the calf muscles than the thigh muscles. A greater decrease in calf muscle mass was related to suppression of translation regulators. Meanwhile, a decrease in endurance of the calf muscles was associated with mitochondrial dysregulation. Most large-scale changes in the transcriptome did not translate into changes in the proteome. However, changes in the RNA levels played a dominant role in regulating expression of specific proteins, such as mitochondrial biogenesis regulators, whereas for others (proteins of ribosome and exosome, etc.) this factor played little or no role. Our findings provide a foundation for developing targeted approaches to counteract the negative effects of disuse on different muscles.

ORGANISM(S): Homo sapiens

PROVIDER: GSE310858 | GEO | 2026/05/19

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Low-intensity neuromuscular electrical stimulation (NMES) is often used as an alternative to exercise and high-intensity electrical stimulation to prevent the loss of muscle mass, strength, and endurance in spaceflight and in patients with severe chronic diseases. This study investigated the effects of a one-week disuse, both with and without low-intensity neuromuscular electrical stimulation – a safe (non-traumatic) approach to prevent the loss of muscle mass, on the functional capacities and gene expression in thigh and calf muscles. This study assessed the efficiency of low-intensity (~10% of maximal voluntary contraction) electrical stimulation in preventing the negative effects of 7-day disuse (dry immersion without [see a related dataset GSE271607] and with daily stimulation) on the strength and aerobic performance of the ankle plantar flexors and knee extensors, mitochondrial function in permeabilized muscle fibers, and the proteomic (quantitative mass spectrometry-based analysis) and transcriptomic (RNA-sequencing) profiles of the soleus muscle and vastus lateralis muscle. Application of electrical stimulation during dry immersion prevented a decrease in the maximal strength and a slight reduction in aerobic performance of the knee extensors, as well as a decrease in maximal (intrinsic) ADP-stimulated mitochondrial respiration and changes in the expression of genes encoding mitochondrial, extracellular matrix, and membrane proteins in the vastus lateralis muscle. In contrast, for the ankle plantar flexors/soleus muscle, electrical stimulation had a positive effect only on maximal mitochondrial respiration, but slightly accelerated the decline in the maximal strength and muscle fiber cross-sectional area, which appears to be related to the activation of inflammatory genes. The data obtained open up broad prospects for the use of low-intensity electrical stimulation to prevent the negative effects of disuse for “mixed” muscles, meanwhile, the optimization of the stimulation protocol is required for “slow” muscles.

Dataset Information

Different effects of 3-week disuse on the phenotype and gene expression of the calf and thigh muscles

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets