ChIP-seq profiling of RNA polymerease III in budding yeast.

ABSTRACT: The genes transcribed by RNA polymerase (Pol) III of yeast are mostly found at sites of high transcription, genomic fragility and replication fork stalling. Transcription terminator-dependent facilitated recycling of Pol III on naked DNA templates boosts transcription in vitro. Recycling on the same template directly influences the reinitiation frequency and hence transcriptional output. Its operability in vivo and on physiological template, the chromatin in vitro, is not known. Using recycling-deficient Pol III or terminator-deficient templates, we found that apart from defining the 3’-end of the transcribed DNA, the terminator is essential for coupling facilitated Pol III recycling to the chromatin remodeling, resulting in higher than the naked DNA level transcription. Mechanistic details of the full activation process revealed three linked steps, anti-repression, chromatin remodeling and Pol III recycling. In normal cells this terminator-facilitated Pol III recycling serves as mechanism for meeting high transcription demands of genes, which may be applicable to even cancerous cells with highly elevated Pol III transcription.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE311094 | GEO | 2026/08/19

REPOSITORIES: GEO