Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Sensitive CAR T cells redefine targetable CD70 expression in solid tumors [ATAC-seq]

ABSTRACT: CD70 expression in solid tumors is heterogeneous. We demonstrate that cells appearing CD70-negative retain ultra-low levels of CD70 expression. These cells that appear negative by conventional detection methods. Bulk ATAC-seq analysis of FACS-sorted CD70⁻ and CD70⁺ tumor populations revealed differential chromatin accessibility, indicating epigenetic regulation of CD70 expression in both ccRCC and HGSOC models.

ORGANISM(S): Homo sapiens

PROVIDER: GSE311096 | GEO | 2026/02/26

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

CUT&RUN profiling of EZH2, H3K27me3, and H3K4me3 enrichment in FACS-sorted CD70⁻ and CD70⁺ tumor cells from untreated ex vivo ccRCC and HGSOC patient-derived xenografts.

Project description:CD70 expression in solid tumors is heterogeneous. We demonstrate that cells appearing CD70-negative retain ultra-low levels of CD70 expression. These cells that appear negative by conventional detection methods. Bulk ATAC-seq analysis of FACS-sorted CD70⁻ and CD70⁺ tumor populations revealed differential chromatin accessibility, indicating epigenetic regulation of CD70 expression in both ccRCC and HGSOC models. CUT&RUN profiling of EZH2, H3K27me3, and H3K4me3 in FACS-sorted CD70⁻ and CD70⁺ tumor cells derived from ccRCC and HGSOC PDXs revealed enrichment of differential repressive and activating histone modifications.

2026-02-26 | GSE311098 | GEO

Sensitive CAR T cells redefine targetable CD70 expression in solid tumors [Multiomics]

Project description:Overcoming tumor antigen heterogeneity in solid tumors is a major challenge for cancer immunotherapies including chimeric antigen receptor (CAR) T cells. Unlike CD19 for B-cell malignancies, no target with pan-cellular expression in solid tumors and absence in normal vital cells has been identified. CD70 is a potential candidate, confined to immune cell subsets and aberrantly expressed in many solid tumors, albeit heterogeneously. We find that CD70 expression in heterogeneous tumors is not binary but ranges from high to very low, appearing negative. We show that CD70-heterogeneous tumors are efficiently eliminated by highly sensitive CD70 receptors where prototypic CAR T cells fail. We further identify an epigenetic signature that predicts targetable expression. These findings provide a potential strategy to treat a broad range of solid tumors.

2026-02-26 | GSE306275 | GEO