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A Complete Spatial Map of Mouse Retinal Ganglion Cells Reveals Density and Gene Expression Specializations [RGC_CrossSection]

ABSTRACT: Retinal ganglion cells (RGCs) transmit visual information from the eye to the brain. In mice, several RGC subtypes show nonuniform spatial distributions, potentially mediating specific visual functions. However, the full extent of RGC specialization remains unknown. Here, we used en-face cryosectioning, spatial transcriptomics, and machine learning to map the spatial distribution of all RGC subtypes identified in previous single-cell studies. While two-thirds of RGC subtypes were evenly distributed, others showed strong biases toward ventral or dorso-temporal regions associated with sky vision and the area retinae temporalis (ART), the predicted homolog of the area centralis. Additionally, we observed unexpected spatial variation in gene expression within several subtypes along the dorso-ventral axis or within vs. outside the ART, independent of RGC density profiles. Finally, we found limited correlations between the gene profiles of the ART and the primate macula, suggesting divergent specialization between the mouse and primate central vision. Pre-dissected regional samples from wild-type mouse retinas cross-sectioned at 20 μm followed by 10x Xenium spatial transcriptomics with the 5000-gene pan-tissue panel. Data processed with Baysor segmentation and CuttleNet cell type prediction as detailed in github.com/sbudoff/MappingAllMurineRGCs

ORGANISM(S): Mus musculus

PROVIDER: GSE311369 | GEO | 2025/12/10

REPOSITORIES: GEO

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