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Protocol for Quantifying Silent Ribosome Induction using Polysome Profiling

ABSTRACT: Translationally silent ribosomes have become an important focus in RNA biology, yet their quantification remains challenging. Lacking mRNAs, they represent a hibernating ribosome state. This protocol outlines how to measure silent ribosome induction under diverse conditions by analyzing RNA from polysome-profiling fractions in yeast and mammalian cells, using RNA-seq with removal of anomalously amplified rRNAs and qPCR for validation.

ORGANISM(S): Mus musculus

PROVIDER: GSE311393 | GEO | 2025/11/26

REPOSITORIES: GEO

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