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Activation of the aryl hydrocarbon receptor in mature adipocytes does not impact diet-induced obesity in mice

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE311599 | GEO | 2025/12/22

REPOSITORIES: GEO

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Activation of the aryl hydrocarbon receptor in mature adipocytes does not impact diet-induced obesity in mice [scRNA-seq]

Project description:Background: Despite rising awareness and advances in the field, the prevalence of obesity continues to increase. Current weight-loss treatments are often ineffective long-term, and some are associated with serious side effects. For this reason, alternative pathways need to be explored in order to identify novel pharmacological targets for the treatment of obesity. In this study, we aim to investigate the role of the ligand-activated transcription factor, aryl hydrocarbon receptor (AHR), in the development of obesity and metabolic syndrome. Methods & Results: Using global and adipocyte-specific Ahr knock-out mouse models, we show that even though global Ahr deletion has some protective effects from diet-induced obesity and metabolic syndrome, adipocyte-specific deletion of Ahr has no effect. Furthermore, using a variety of techniques to isolate mature adipocytes, such as flotation, in-vitro differentiation, and translating ribosome affinity purification, we show no evidence that Ahr is expressed in mature white adipocytes. Finally, using single-cell RNA sequencing on cells from a Cyp1a1 fate reporter mouse line, in which eYFP can be used as a readout of AHR activation, we show that the AHR pathway is active in adipose stem and precursor cells. Using bulk RNA sequencing on adipocyte precursors stimulated with an AHR ligand in-vitro, we show evidence suggesting that Ahr may be involved in the control of adipocyte differentiation. Conclusions: Our study indicates that Ahr may play an important role in adipocyte precursors, particularly in the initiation of adipocyte differentiation. We suggest that these results warrant further research in this mechanism in the context of obesity.

2025-12-22 | GSE311598 | GEO

Activation of the aryl hydrocarbon receptor in mature adipocytes does not impact diet-induced obesity in mice [RNA-seq]

2025-12-22 | GSE311153 | GEO

Activation of the aryl hydrocarbon receptor in mature adipocytes does not impact diet-induced obesity in mice

Project description:Activation of the aryl hydrocarbon receptor in mature adipocytes does not impact diet-induced obesity in mice

| PRJNA1369970 | ENA

Activation of the aryl hydrocarbon receptor in mature adipocytes does not impact diet-induced obesity in mice [RNA-seq]

Project description:Activation of the aryl hydrocarbon receptor in mature adipocytes does not impact diet-induced obesity in mice [RNA-seq]

| PRJNA1368759 | ENA

Activation of the aryl hydrocarbon receptor in mature adipocytes does not impact diet-induced obesity in mice [scRNA-seq]

Project description:Activation of the aryl hydrocarbon receptor in mature adipocytes does not impact diet-induced obesity in mice [scRNA-seq]

| PRJNA1369975 | ENA

Activation of aryl hydrocarbon receptor

Project description:VAF347 is a low molecular weight compound which inhibits allergic lung inflammation in vivo. This effect is likely due to a block of dendritic cell (DC) function to generate pro-inflammatory T-helper (Th) cells since VAF347 inhibits IL-6, CD86 and HLA-DR expression by human monocyte derived DC, three relevant molecules for Th-cell generation. Here we demonstrate that VAF347 interacts with the aryl hydrocarbon receptor (AhR) protein resulting in activation of the AhR signaling pathway. Functional AhR is responsible for the biological activity of VAF347 since, i) other AhR agonists display an identical activity profile in vitro, ii) gene silencing of wild type AhR expression or forced over-expression of a trans-dominant negative AhR ablates VAF347 activity to inhibit cytokine induced IL-6 expression in a human monocytic cell line and iii) AhR deficient mice are resistant to the compound’s ability to block allergic lung inflammation in vivo. These data identify the AhR protein as key molecular target of VAF347 and its essential role for mediating the anti-inflammatory effects of the compound in vitro and in vivo. Keywords: effect of compound

2008-02-12 | GSE10463 | GEO