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Exon level expression profiling of mutant U2AF35 transduced HeLa cells

ABSTRACT: In this study, to obtain the biological impact of the mutated U2AF35, HeLa cells were retrovirally transduced with either mock, wild-type or S34F mutant of U2AF35, and Exon array was performed.

ORGANISM(S): Homo sapiens

PROVIDER: GSE31171 | GEO | 2011/09/12

SECONDARY ACCESSION(S): PRJNA154081

REPOSITORIES: GEO

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Exon level expression profiling of mutant U2AF35 transduced HeLa cells

Project description:In this study, to obtain the biological impact of the mutated U2AF35, HeLa cells were retrovirally transduced with either mock, wild-type or S34F mutant of U2AF35, and Exon array was performed. Exon level analysis was performed for mock, wild-type U2AF35 or S34F mutant transduced HeLa in triplicate.

2011-09-11 | E-GEOD-31171 | biostudies-arrayexpress

Expression analysis of mutant U2AF35 transduced cells

2011-09-12 | GSE31172 | GEO

Expression analysis of mutant U2AF35 transduced cells

Project description:In this study, to obtain the biological impact of the mutated U2AF35, HeLa and TF-1 cells were retrovirally transduced with either mock, wild-type or S34F mutant of U2AF35, and Expression array was performed. Expression analysis was performed for mock, wild-type U2AF35 or S34F mutant transduced HeLa and MDS-derived TF-1 cell line in triplicate.

2011-09-11 | E-GEOD-31172 | biostudies-arrayexpress

Expression profile of mutant U2AF35 transduced cells

Project description:This SuperSeries is composed of the following subset Series: GSE31171: Exon level expression profiling of mutant U2AF35 transduced HeLa cells GSE31172: Expression analysis of mutant U2AF35 transduced cells Refer to individual Series

2012-04-09 | E-GEOD-31173 | biostudies-arrayexpress

U2AF35(S34F) Promotes Transformation by Directing Aberrant ATG7 Pre-mRNA 3’ End Formation

Project description:We sequenced mRNA from Ba/F3 cells with U2AF35(S34F) expressing or parental Ba/F3

2016-05-01 | GSE63404 | GEO

Expression analysis of mock- or RAD21-transduced Kasumi1 cells

Project description:We recently identified recurrent mutations of cohesin complex in myeloid neoplasms through whole-exome sequencing analysis. RAD21 is one of the main components of the cohesin complex. In this study, to investigate the biological impact of wild-type RAD21 on Kasumi1 cells harboring RAD21 mutation, Kasumi1 cells were retrovirally transduced with either mock or wild-type RAD21, and expression array was performed.

2013-07-16 | GSE47641 | GEO

Expression analysis of mock- or RAD21-transduced Kasumi1 cells

2013-07-16 | E-GEOD-47641 | biostudies-arrayexpress

Expression data from mutant FoxP3-transduced CD4 T cells

Project description:FoxP3 is a central regulator of immunological tolerance, controlling the development and function of regulatory T (Treg) cells. To dissect the complex processes orchestrated by FoxP3, we investigated impacts of three autoimmune disease-associated missense FoxP3 mutations in mice. As a initial approach, we retrovirally transduced naïve conventional T (Tconv) cells with WT or mutant (A384T and R397W) FoxP3 and analyzed the gene expression profiles of the transduced T cells.

2017-08-01 | GSE89645 | GEO

Gene expression profiling of mouse HSPCs retrovirally transduced with Plzf

Project description:We have previously found the immortalization of mouse HSPCs retrovirally transuced with Plzf. To investigate the moleular mehcanism of the immortalization, we preliminarily performed comprehensive gene expression profiling of Plzf-transduced KSL and myeloid progenitor-enriched (MP) cells using cDNA microarray analysis.

2017-04-26 | GSE84771 | GEO

Exon arrays from HeLa cells transfected with GFP-ZRANB2 vector

Project description:Human ZRANB2 mRNA is alternatively spliced to give two variants with different 3M-bM-^@M-^Y ends. Both isoforms are present in the nucleus of human cells. ZRANB2 binds to mRNA, as well as the essential splicing factors U170K and U2AF35, and the novel splicing component SFRS17A (formerly known as XE7). It has been shown to alter splicing patterns of Tra2M-NM-21 minigene primary transcripts in a dose-dependent manner. It is still unclear what role ZRANB2 plays in the regulation of alternative splicing. To determine the endogenous transcripts that are targeted by ZRANB2 at the genome wide level, we decided to perform exon microarray studies and analyzed the suitability of this method to examine splicing differences in human cells overexpressing a splicing factor. GFP-ZRANB2 construct containing isoform 2 (short form, SF) of ZRANB2 was used. This construct includes exon 1 through to alternative exon 10. Isoform 2 was chosen since it has been shown previously to affect alternative splicing of a Tra2M-NM-21 minigene. HeLa cells were transfected with GFP-ZRANB2, control vector GFP or were left untransfected. RNA was extracted using a Qiagen RNA extraction kit. Experiments were run in 5 replicates.

2012-12-15 | E-GEOD-32932 | biostudies-arrayexpress

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