Transcriptome profiling of Hansenula polymorpha in response to sulfur starvation and cadmium stress
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ABSTRACT:
ORGANISM(S): Ogataea angusta
PROVIDER: GSE31197 | GEO | 2014/07/31
SECONDARY ACCESSION(S): PRJNA144747
REPOSITORIES: GEO
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Project description:Transcriptome profiling analysis of the Hansenula polymorpha MET4 gene deletion strain have been carried out to obtain comprehensive information on the HpMet4p-mediated regulatory networks in association with the cadmium (Cd) detoxification and sulfur regulation in H. polymorpha. Total RNA samples were collected from H. polymorpha wild type, and HpMET4 deletion strain, under sulfur starvation or Cd (0.6 mM) stress conditions. The differential fluorescence intensities of each RNA sample were measured after labeling with Cy3 or Cy5. For all analyses, we performed dye swapping experiments to avoid dye bias. Thus, four intensity values were generated for each ORF and averaged for analysis.
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Project description:The only group of organisms in which a biological function for cadmium has been shown is the diatoms, which are unicellular phytoplankton. Yet diatoms exhibit similar sensitivity to Cd as other groups of phytoplankton. We have investigated responses of Cd on molecular, metabolic and physiological levels in the diatom Phaeodactylum tricornutum. P. tricornutum apparently has a high tolerance to Cd; only minor responses were observed on growth, pigment and transcriptional changes at cadmium concentrations of 123 µg/l. No significant changes in chlorophyll and xanthophyll levels were observed, and the very few transcripts affected strongly indicate that the cells were able to respond to the increased Cd2+ levels without changing proteins levels. At 10 times this concentration, 1230 µg/l, a much clearer response was observed, including transcripts encoding proteins involved in metal transport, cell signalling and detoxification processes. Our results point towards putative pathways for the removal or detoxification of Cd and its metabolites, as well as a possible Cd uptake mechanism. We predict that ATPase5-1B is involved in removal of Cd by pumping Cd2+ ions out of the cell, whereas VIT1/CCC1 sequesters Cd2+ in the vacuole. Cultures of Phaeodactylum tricornutum were treated with two concentrations of Cd2+, 123 µg/l and 1230 µg/l. The reference samples were grown in artificial seawater base AK modified with added f/2-medium nutrients. Three biological replicates were harvested for all samples.
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Project description:Cadmium is toxic and intricate pathways linked to metallothioneins (MT) drive the detoxification process. Whist the mechanisms are well understood in mammalian systems, detailed knowledge is still elusive in invertebrates (which notably differ to mammalian systems). The model nematode Caenorhabditis elegans is ideally suited for assessing metallothionein mediates detoxification in invertebrates as is relatively short-lived, can be easily exposed and its genome is fully sequenced and widely annotated. The aim of the experiment was to identify new MT mediated target genes involved in Cd toxicosis.
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