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Glutathione-induced elicitation of defense responses in Lotus japonicus.

ABSTRACT: To identify transcription factors involved in the production of defense-related phytochemicals through co-expression studies.

ORGANISM(S): Lotus japonicus

PROVIDER: GSE31240 | GEO | 2011/09/16

SECONDARY ACCESSION(S): PRJNA146043

REPOSITORIES: GEO

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Glutathione-induced elicitation of defense responses in Lotus japonicus.

Project description:To identify transcription factors involved in the production of defense-related phytochemicals through co-expression studies. The changes in gene expression following GSH elicitation of leaves was followed over a time course. Four biological replicates of the unelicited samples and three biological replicates were used for each time point.

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Project description:Phosphopeptides were purified using Titansphere. Before MS analysis, phosphopeptides were desalted using StageTips with C18 Empore disk membranes. Phosphopeptides were analysed by Orbitrap MS.The obtained MS and MS/MS spectra data were searched against Kazusa DNA Research Institute published lotus EST database using the MASCOT program (FDR<1%).

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