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Gene expression profiling of Escherichia coli isolates from aging colonies (Series 1)

ABSTRACT: This series presents normalized gene expression profiles of three Escherichia coli K-12 MG1655-derived strains (pygYFP, Y5, and Y6). Strains Y5 and Y6 were originally isolated from 3-week-old aging colonies, but RNA for transcriptomic profiling was extracted from 7-day-old colonies regrown under identical conditions. All samples were hybridized to the NimbleGen GPL9088 one-color microarray platform, and processed values correspond to RMA-normalized, non-log2 intensities. These data compare the parental strain to two evolved isolates carrying distinct regulatory and stress-response mutations, and can be used to study diversification and adaptive strategies emerging in aging bacterial colonies.

ORGANISM(S): Escherichia coli Escherichia coli str. K-12 substr. MG1655

PROVIDER: GSE312419 | GEO | 2026/03/10

REPOSITORIES: GEO

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Project description:This series presents normalized gene expression profiles of three Escherichia coli K-12 MG1655-derived strains (pygYFP, Y5, and Y6). Strains Y5 and Y6 were originally isolated from 3-week-old aging colonies, but RNA for transcriptomic profiling was extracted from 7-day-old colonies regrown under identical conditions. All samples were hybridized to the NimbleGen GPL9088 one-color microarray platform. Processed values correspond to RMA-normalized, non-log2 intensities. Raw .pair files and the processed matrix for these samples are provided. These data complement Series 1 and constitute a second biological replicate (B2) for transcriptomic analysis of the parental strain and two evolved isolates carrying distinct mutations affecting regulatory and stress-response pathways in aging bacterial colonies.

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