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Transcriptional profile of primary human lung organoid-derived air-liquid interface (ALI) cultures

ABSTRACT: Innate defences of the respiratory epithelium are the first barrier against incoming respiratory viruses. To understand the contribution of both basal (tonic) and induced interferon (IFN) to antiviral defences in a physiologically relevant system, we established air-liquid interface (ALI) cultures of primary human bronchial epithelium (HBE) and small airway epithelium (HSE). Via an organoid intermediate stage, the limited healthy donor material was expanded while preserving stemness and subsequently differentiated. We here perform bulk RNA-seq to characterize the transcriptomes and cellular diversity of our ALI cultures.

ORGANISM(S): Homo sapiens

PROVIDER: GSE312700 | GEO | 2026/05/05

REPOSITORIES: GEO

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