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Single-cell RNA-seq of colonic tumors from epithelial HO-1 knockout and control mice in a colitis-associated cancer model

ABSTRACT: This dataset contains single-cell RNA sequencing profiles of colonic tumor cells isolated from mice with intestinal epithelial-specific deletion of Hmox1 and littermate controls. Tumors were generated using the azoxymethane-dextran sodium sulfate (AOM-DSS) model of colitis-associated cancer. Following enzymatic dissociation and live cell sorting, single-cell libraries were prepared using the 10x Genomics Chromium platform and sequenced to characterize transcriptional programs within the tumor microenvironment. The dataset was designed to examine genotype-dependent shifts in epithelial transcriptional programs within the tumor microenvironment, with a focus on stress-adaptive responses, iron metabolism, and oxidative stress regulation.

ORGANISM(S): Mus musculus

PROVIDER: GSE312733 | GEO | 2025/12/12

REPOSITORIES: GEO

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