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Whole-genome bisulfite sequencing (WGBS) of wild-type and Uhrf1-/- ESCs

ABSTRACT: UHRF1 is a chromatin-binding protein essential for maintaining DNA methylation and histone modification states, yet its integrated role in vivo remains incompletely understood. To define its function, we generated conditional Uhrf1 knockout embryonic stem cells (ESCs) and embryos. Uhrf1⁻/⁻ ESCs exhibited near-complete loss of 5mC and 5hmC but maintained pluripotency, whereas Uhrf1-null embryos developed normally until E8.5 and then failed to further develop by E9.5.

ORGANISM(S): Mus musculus

PROVIDER: GSE312765 | GEO | 2026/03/21

REPOSITORIES: GEO

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Uhrf1 loss alters Ctcf-mediated topological organization during early mouse embryogenesis

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