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Single-Cell RNA Sequencing Analysis of Kidney Organoids derived in 2D or 3D from SCTi004-A hiPSC line using the STEMdiff™ Kidney Organoid Kit

ABSTRACT: Kidney organoids differentiated from human pluripotent stem cells are complex structures that capture glomerular, tubular and mesenchymal cell types of the developing human kidney. The STEMdiff™ Kidney Organoid Kit is a serum-free cell culture medium system for the efficient and reproducible generation of kidney organoids in 2D using a simple, 18 day, two-stage differentiation protocol. We recently also showed compatibility of this kit for the generation of 3D organoids using single cell dissociation and re-aggregation on Day 7. Here we evaluate kidney organoids derived from the commercially available hiPSC line SCTi004-A with the STEMdiff™ Kidney Organoid Kit for their cellular diversity and transcriptomic profiles. Datasets were created for kidney organoids differentiated in the 2D and 3D format.

ORGANISM(S): Homo sapiens

PROVIDER: GSE312980 | GEO | 2025/12/09

REPOSITORIES: GEO

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