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Effects of dietary human milk oligosaccharides (HMO) and Trichuiris muris (Tm) infection on the caecum transcriptome in mice

ABSTRACT: We examined how the adminsiration of prebiotic carbohyrdates (HMOs) and pathogen infection (Tm) interacted to modulate intestinal transcriptomic responses.

ORGANISM(S): Mus musculus

PROVIDER: GSE312987 | GEO | 2026/04/01

REPOSITORIES: GEO

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Effects of dietary human milk oligosaccharides (HMO) and Trichuiris muris (Tm) infection on the caecum transcriptome in mice

Project description:Effects of dietary human milk oligosaccharides (HMO) and Trichuiris muris (Tm) infection on the caecum transcriptome in mice

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Project description:In this study, we quantitated the disappearance of intact HMOs and characterized the glycan digestion products in the gut that are produced by the action of microbial enzymes on HMOs and glycoconjugates from breast milk. Oligosaccharides from fecal samples of exclusively breast-fed infants were extracted and profiled using nanoLC-MS. Intact HMOs were found in the fecal samples, additionally, other oligosaccharides were found corresponding to degraded HMOs and non-HMO based compounds. The latter compounds were fragments of N-glycans released through the cleavage of the linkage to the asparagine residue and through cleavage of the chitobiose core of the N-glycan.

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Project description:Bifidobacterium longum subsp. infantis (B. infantis) colonizes the infant gut microbiome with a 43-kb gene cluster that enables human milk oligosaccharide (HMO) utilization. Although there is relative genomic homogeneity in this regard, previous observations suggest that B. infantis strains may differ in their utilization phenotype. To test this hypothesis, a panel of B. infantis strains were evaluated for their ability to utilize pooled HMOs to yield differential phenotypes including biomass accumulation, HMO consumption glycoprofile, end-product secretion, and global transcriptomes. Two strains (ATCC 15697 and UMA301) efficiently consumed several HMO isomers/anomers that exhibit degrees of polymerization (DP) ³ 4. These same strains partially consumed the smaller DP HMOs including fucosyllactose and lactodifucotetraose isomers/anomers. In contrast, UMA299 efficiently utilized fucosylated small molecular weight HMOs (DP<4), and accumulated greater biomass on purified 2´FL with significantly higher 1,2-propanediol production. This study identifies several strain-dependent features in HMO utilization phenotypes that are consistent with metabolic variation within a bifidobacterial-dominated infant-gut microbiome.

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Project description:The factors that govern the retention and abundance of specific microbial lineages within a developing intestinal microbiota remain poorly defined. Human milk oligosaccharides consumed by nursing infnats pass undigested to the distal gut where they may be consumed by microbes. We investigated the transcriptional response of Bacterides fragilis, a prominent gut resident, to the presence of HMOs. In vitro transcriptional profiles of Bacteroides fragilis obtained from biological duplicate cultures taken at middle log phase in minimal media glucose (MM-Glu) and in minimal media with human milk oligosaccharides (MM-HMO).

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2012-09-30 | GSE32207 | GEO

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Project description:Mice were fed either a semi-synthetic diet ('SSD'), or the same diet supplemented with the fiber inulin ('inulin'). Mice were then infected with Trichuris muris ('Tm').

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