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Murine Myoblast C2C12 Cells: Control vs. Mybbp1a Knockdown

ABSTRACT: Transcriptional profiling of mouse myoblast cells comparing control vs. Mybbp1a knockdown. Stable clones of C2C12 cells harboring control or Mybbp1a-targeting shRNA were established and further pooled for analysis. Goal was to determine, based on the effects of Mybbp1a depletion on global gene expression, candidate downstream target genes of Mybbp1a, a putative transcriptional co-repressor.

ORGANISM(S): Mus musculus

PROVIDER: GSE31319 | GEO | 2011/08/10

SECONDARY ACCESSION(S): PRJNA146091

REPOSITORIES: GEO

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Murine Myoblast C2C12 Cells: Control vs. Mybbp1a Knockdown

Project description:Transcriptional profiling of mouse myoblast cells comparing control vs. Mybbp1a knockdown. Stable clones of C2C12 cells harboring control or Mybbp1a-targeting shRNA were established and further pooled for analysis. Goal was to determine, based on the effects of Mybbp1a depletion on global gene expression, candidate downstream target genes of Mybbp1a, a putative transcriptional co-repressor. Two-condition experiment, control vs. Mybbp1a knockdown C2C12 cells (mixed stable clones). Biological replicates: 2.

2011-08-09 | E-GEOD-31319 | biostudies-arrayexpress

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C2C12 murine myoblast cell line and 48 h 10 uM BIO treated C2C12 cellulate

Project description:In urodele amphibians, limb regeneration involves the dedifferentiation of muscle myotubes into single cells that may acquire pluripotent potential. We have employed small molecules (myoseverin and BIO) to attempt to reproduce this behavior in mammalian muscle culture. C2C12 myotubes derived from the C2C12 myoblast cell line were induced to undergo cellularization by myoseverin treatment, which destabilizes tubulin filaments. The GSK-3 inhibitor, BIO, was then used to induce dedifferentiation. Induce neuron formation; the cells were incubated with 250 nM reversine for 48 h, and neural induction media (DMEM/F12 supplemented with N2 (Invitrogen)) and 1.5 uM all-trans retinoic acid for 7 days. C2C12 murine myoblast cell line and 48 h 10 uM BIO treated C2C12 cellulate (derived by 20 M myoseverin treatment for 48 h)

2011-12-08 | GSE34238 | GEO

Mus musculus

Project description:Murine Myoblast C2C12 Cells: Control vs. Mybbp1a Knockdown

| PRJNA146091 | ENA

C2C12 murine myoblast cell line and 48 h 10 uM BIO treated C2C12 cellulate

2011-12-08 | E-GEOD-34238 | biostudies-arrayexpress

Gene Expression Profiling of C2C12 Myoblast Differentiation

Project description:In this study, the C2C12 cell line, a model used to study myogenesis and regeneration, was allowed to differentiate from myoblast precursor cells to myotubes. Cells were harvested at 4 different timepoints to perform gene expression profiling. We identified genes that were up-regulated and down-regulated during the differentiation process.

2010-07-05 | GSE19968 | GEO

Msx1 ChIP-Seq from C2C12 murine myoblast cells expressing Flag-Msx1 protein

Project description:The spatial and temporal control of gene expression during development requires the concerted actions of sequence-specific transcriptional regulators and epigenetic chromatin modifiers, which are thought to function within precise nuclear compartments. However, how these activities are coordinated within the dynamic context of the nuclear environment is still largely unresolved. Here we show that transcriptional repression by the Msx1 homeoprotein coordinates recruitment of Polycomb to genomic targets with localization to the nuclear periphery. We used genome-wide ChIP-Seq analyses to identify genomic binding sites for Msx1 in C2C12 murine myoblast cells. C2C12 myoblast cells were seeded at a density of 2.5 x 10^5 cells / 10-cm plate one day prior to infection, and infected with a retrovirus containing a Flag-Msx1 construct for two consecutive days. The resulting cells were then crosslinked with formaldehyde, and DNA was enriched by chromatin immunoprecipitation (ChIP) with an anti-Flag antibody and analyzed by Solexa sequencing. Enriched regions were identified using a Poissonian background model, and were further compared to an additional background of sequences from a sample of Flag-immunoprecipitated DNA from C2C12 cells infected with an empty vector to determine enrichment. ChIP was performed using an antibody against the Flag epitope (Sigma M2, F3165).

2011-08-17 | E-GEOD-26711 | biostudies-arrayexpress

Msx1 ChIP-Seq from C2C12 murine myoblast cells expressing Flag-Msx1 protein

2011-08-17 | GSE26711 | GEO

siRNA profiling of mouse myoblast C2C12 cells reveals PPARbeta/delta dependent differentiation

Project description:siRNA profiling of mouse myoblast C2C12 cells reveals PPARbeta/delta dependent differentiation

2012-02-01 | E-MTAB-528 | biostudies-arrayexpress

Transcription profiling of mouse undifferentiated C2C12 myoblast to 4 day differentiated myotubes

Project description:Comparison of undifferentiated C2C12 myoblast to 4 day differentiated myotubes. Experiment Overall Design: this experiment include 2 samples and 6 replicates

2008-06-12 | E-GEOD-3243 | biostudies-arrayexpress

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