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Single-cell transcriptomic profiling of c-Kit⁺ HSPCs in a KrasG12D/+ mouse model of JMML

ABSTRACT: We performed single-cell RNA sequencing (scRNA-seq) to characterize hematopoietic stem and progenitor cells (HSPCs) in a KrasG12D/+ mouse model of juvenile myelomonocytic leukemia (JMML). C-Kit⁺ HSPCs were enriched from the bone marrow of 6-week-old KrasG12D/+ and wild-type (WT) mice using CD117 MicroBeads and processed using the 10x Genomics Chromium Single Cell 3′ v3 platform. Libraries were sequenced on an Illumina NovaSeq 6000 system. Raw data were processed with Cell Ranger and analyzed using Seurat for quality control, dimensionality reduction, and clustering. HSPC subsets, developmental trajectories, and pathway activities were then analyzed to characterize leukemic alterations.

ORGANISM(S): Mus musculus

PROVIDER: GSE313553 | GEO | 2026/03/31

REPOSITORIES: GEO

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Single-cell transcriptomic profiling of c-Kit⁺ HSPCs in a KrasG12D/+ mouse model of JMML

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