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Single-nucleus RNA sequencing and functional studies of acute methamphetamine-induced cognitive impairment

ABSTRACT: Cognitive impairment is a hallmark phenotype of methamphetamine (Meth) abuse, yet the cellular mechanisms driving these deficits remain poorly understood. This study aims to investigate the cellular composition, gene expression profiles, key signaling pathways, intercellular communication networks, and transcription factor activity in hippocampal tissues from control mice and those acutely exposed to Meth, thereby elucidating the mechanistic underpinnings of Meth-induced neurotoxicity. Single-nucleus RNA sequencing (snRNA-seq) was employed to investigate the transcriptomes of nuclei isolated from the hippocampus of acute Meth-treated and control mice. Our study provides a systemic understanding of the transcriptome alterations induced by Meth exposure in mouse hippocampus, which may offer clues for therapeutic strategies against acute Meth exposure.

ORGANISM(S): Mus musculus

PROVIDER: GSE313595 | GEO | 2026/04/01

REPOSITORIES: GEO

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