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DNMT3L crosstalk with piRNA biogenesis in postnatal spermatogonial progenitor cells

ABSTRACT: We analyzed small RNAs expression profiles of defferent developmental stage (0 dpp and 8 dpp) to investigate the potential role of DNMT3L in spermatogonial stem cell. We observed that relative proportion of microRNA and piRNA were similar betwee wild type and Dnmt3l knouckout mice. However, piRNA origin and composition significantly differed in 8 ddp Dnmt3l-deficiency samples, and genomic location of predicted precursors remarkably shitfted as well.

ORGANISM(S): Mus musculus

PROVIDER: GSE313665 | GEO | 2026/03/13

REPOSITORIES: GEO

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DNMT3L promotes quiescence in postnatal spermatogonial progenitor cells

Project description:We analyzed transcriptome profiles of postnatal germ cells and demonstrated that DNMT3L is important for spermatogonial stem/progenitor cell development Examination of wild-type and Dnmt3l knockout smaples in postnatal male germ cells

2014-07-03 | E-GEOD-54411 | biostudies-arrayexpress

DNMT3L promotes quiescence in postnatal spermatogonial progenitor cells

Project description:We analyzed transcriptome profiles of postnatal germ cells and demonstrated that DNMT3L is important for spermatogonial stem/progenitor cell development

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Defective germline reprogramming rewires the spermatogonial transcriptome

Project description:Defective germline reprogramming in Miwi2- and Dnmt3l-deficient mice results in the failure to reestablish transposon silencing, meiotic arrest and progressive loss of spermatogonia. Here we sought to understand the molecular basis for this spermatogonial dysfunction. Through a combination of imaging, conditional genetics and transcriptome analysis, we demonstrate that germ cell elimination in the respective mutants arises due to defective germline reprogramming rather than a function for the respective factors within spermatogonia. In both Miwi2-/- and Dnmt3l-/- spermatogonia the intracisternal-A particle (IAP) family of endogenous retroviruses is de-repressed, but in contrast to meiotic cells DNA damage is not observed. Instead we find that unmethylated IAP promoters rewire the spermatogonial transcriptome by driving expression of neighboring host genes. In summary, defective reprogramming deregulates the spermatogonial transcriptome that may underlie spermatogonial dysfunction.

2018-03-09 | E-MTAB-6202 | biostudies-arrayexpress

Dnmt3L-histone H3 binding is necessary for male fertility and non-CG methylation

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2015-01-14 | E-GEOD-58066 | biostudies-arrayexpress

Transposon-derived piRNA production in pre-natal and post-natal stages, in Dnmt3L and Miwi2 mutant tetses [small RNA-seq]

Project description:We examined the kinetics of production of mRNAs and small RNAs derived from transposable elements during mouse spermatogenesis, in whole gonads of wildtype and DNA methylation-deficient males (Dnmt3L and Miwi2 mutants). We found that in absence of DNA methylation, transposon reactivation is not constitutive but rather occurs in a class- and development-specific manner : both the intensity of reactivation and the number of reactivated transposon classes increased as germ cells progress in meiosis. Moreover, we observed that transposon silencing before meiosis is not due to increased cleavage by the piRNA machinery. In contrast, the burst of transposon transcripts occurring at meiosis in the absence of DNA methylation serve as substrates for increased piRNA production

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Dnmt3L-histone H3 binding is necessary for male fertility and non-CG methylation

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The effect of the Pld6 and Dnmt3l mutations on DNA methylation and expression of retrotransposons in mouse male germ cells

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2017-07-20 | GSE70891 | GEO

Transposon expression kinetics in Dnmt3L-/- developing testes [RNA-seq]

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DNA methylation changes in Dnmt3L-deficient mouse embryonic stem cells (mESCs)

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2018-10-04 | GSE116489 | GEO

Genome-wide analysis of Dnmt3L heterozygous and wildtype spermatocytes and spermatids

Project description:Comparison of gene expression differences between Dnmt3L heterozygous and wildtype pachytene spermatocytes, and similarly between Dnmt3L heterozygous and wildtype round spermatids which were isolated from the Dnmt3L knockout mouse line. This array was conducted to address the hypothesis that Dnmt3L heterozygosity results in deregulated gene expression within spermatocytes and spermatids. Results show that Dnmt3L heterozygosity causes numerous genes to be differentially regulated on a genome-wide level, showing that DNMT3L has an important role in regulating gene expression within these male germ cells. Three preparations each of Dnmt3L purified wildtype spermatocytes, wildtype spermatids, heterozygous spermatids, and two preparations of heterozygous spermatocytes were isolated for a total of 11 samples. Each preparation was made up of cells isolated from 10 mice.

2011-03-01 | E-GEOD-20241 | biostudies-arrayexpress

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