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Spatial transcriptomic profiling of bone marrow from KrasG12D/+ and wild-type mice

ABSTRACT: Spatial transcriptomic profiling was performed on whole bone marrow sections from KrasG12D/+ and wild-type mice. Frozen 10-µm bone marrow sections were processed by Biomarker Technologies Corporation (Beijing, China) following standard spatial transcriptomics procedures, including tissue fixation, H&E staining, permeabilization, reverse transcription, and spatial cDNA library construction. Libraries were sequenced on an Illumina NovaSeq 6000 (PE150). Spot-level gene expression matrices were generated using BSTMatrix, aligned with corresponding bright-field images, and tissue-covered spots were identified for downstream analysis. Spatial mapping revealed spatially resolved patterns of gene expression and cellular organization within bone marrow. These data provide comprehensive spatial transcriptomic information enabling investigation of bone marrow architecture and microenvironmental interactions in Kras-driven JMML.

ORGANISM(S): Mus musculus

PROVIDER: GSE313878 | GEO | 2026/03/31

REPOSITORIES: GEO

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