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BDNF engages pituitary stem cells

ABSTRACT: The development of complex organs relies on the balanced expansion of cells of different lineages in order to establish a functional tissue. In the pituitary gland, maintenance of the adult corticotrope lineage relies heavily on replication of differentiated cells. In contrast in pathological conditions, such as adrenalectomy, that deplete circulating glucocorticoids (Gc), pituitary stem cells are activated to increase the number of corticotropes. In the present work, we used the POMC knockout mouse model (POMC-/-) of Gc deficiency to identify and characterize pre-corticotrope precursors that are normally rare and short lived but accumulate in this model. Differentiation of PSCs into pre-corticotropes is repressed by Gc and requires the lineage-specific transcription factor Tpit. Pre-corticotrope maintenance is dependent on a BDNF autocrine loop that is sensitive to Gc. In addition, BDNF is a critical signal for engagement of pituitary stem cells into proliferation and differentiation during the postnatal expansion of the gland.

ORGANISM(S): Mus musculus

PROVIDER: GSE313979 | GEO | 2026/05/18

REPOSITORIES: GEO

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BDNF engages pituitary stem cells for establishment of the adult gland and for homeostasis of the corticotrope lineage

Project description:The development and maintenance of complex organs rely on the balanced expansion of cells of different lineages to establish a functional tissue. In the pituitary gland, maintenance of the adult corticotrope lineage relies heavily on replication of differentiated cells. In contrast in pathological conditions, such as adrenalectomy that depletes circulating glucocorticoids (Gc), pituitary stem cells (PSC) are activated to increase the number of corticotropes. In the present work, we used the proopiomelanocortin (POMC) knockout mouse model of Gc deficiency to characterize pre-corticotrope precursors that are normally rare and short lived but accumulate in this model. Differentiation of PSCs into pre-corticotropes is repressed by Gc and requires the lineage-specific transcription factor Tpit. Pre-corticotrope maintenance is dependent on a brain-derived neurotropic factor (BDNF) autocrine loop that is sensitive to Gc. In normal development, BDNF is critical for engagement of PSCs into proliferation and differentiation during the postnatal expansion of the gland.

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