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HEK293 cells with control or NFIL3 shRNA treatment

ABSTRACT: Transcriptional profiling with samples from HEK293 cells that were infected with control or NFIL3 shRNA

ORGANISM(S): Homo sapiens

PROVIDER: GSE31426 | GEO | 2012/09/02

SECONDARY ACCESSION(S): PRJNA154169

REPOSITORIES: GEO

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HEK293 cells with control or NFIL3 shRNA treatment

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2012-09-02 | E-GEOD-31426 | biostudies-arrayexpress

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Homo sapiens

Project description:HEK293 cells with control or NFIL3 shRNA treatment

| PRJNA154169 | ENA

Differential requirement for Nfil3 during NK cell development

Project description:Nfil3 has multiple functions during conventional NK cell and thymic NK cell development. Nfil3 controls NK cell development from the earliest committed NK cell progenitor stage. Nfil3-/- prepro NK cells retained T-cell potential due to the failure to repress T cell specific genes. At later stages of NK cell development, Nfil3 regulates the formation of mature NK cells by promoting directly or indirectly the expression of transcription factor Eomes. We also demonstrate that Nfil3 is important for thymic NK cell development whereas Trail+ immature NK cells generated in the bone marrow of neonates and in the adult liver develop independent of Nfil3 function. We used microarrays to analyse the changes in gene expression due to the presence or absence of Nfil3, both in prepro NK cells and ALP cells (all lymphoid progenitors) Samples were obtained on two experimentation dates. Replicate 1 was processed in April 2012, replicate 2 was processed in May 2012.

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RNAseq analysis of BDAA treatment on YFV infected HEK293 cells

Project description:Purpose: to investigate the change in gene expression after BDAA treatment on YFV infected HEK293 cells. Methods: HEK293 cells were cultured; then cells were non-infected/infected with YFV, and then untreat/treat with BDAA. Total RNA were harvested and sent to RNA sequencing. Results: we demonstrated that BDAA treatment of YFV infected HEK293 cells at 18 hpi for 2 h increased expression of a total of 39 cellular genes, 33 of which are inflammatory cytokines, chemokines or ISGs, and the remaining are mostly genes related to NFκB, TNF-α and MAPK signal transduction. Conclusion: BDAA triggers an enhanced activation of RNA sensor-mediated inflammatory cytokine responses, most likely by YFV RNA.

2021-03-04 | GSE168183 | GEO

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