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FACT maintains nucleosomes around promoters and opposes to the spreading of chromatin factors, thereby playing a major role in chromatin architecture [TT-seq]

ABSTRACT: Amounting evidence suggests nucleosome organization is key for higher levels of chromatin packaging. However, studying it in higher eukaryotes is challenging due to the difficulty to manipulate nucleosomes in vivo and reconstitute their large higher-order chromatin domains in vitro. Facilitates chromatin transcription (FACT) is a H2A/B histone chaperone that displaces and re-assembles histones, being important for processes such as transcription. Recent studies report a minor role of FACT in chromatin architecture. Here, we studied chromatin organization upon rapid FACT degradation employing a multidimensional 3C technique with base-pair resolution (MCC-u) and genomic approaches. FACT loss leads to nucleosome depletion around active promoters, nano-scale domain collapse and emergence of larger nucleosome-free regions with increased protein binding. At a larger scale, we detect increased interactions between promoters across topologically associated domains. Thus, we demonstrate FACT plays a major role in chromatin organization and gain insights into the role of nucleosomes in genome architecture.

ORGANISM(S): Mus musculus

PROVIDER: GSE314896 | GEO | 2026/03/30

REPOSITORIES: GEO

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FACT maintains nucleosomes around promoters and opposes to the spreading of chromatin factors, thereby playing a major role in chromatin architecture

Project description:Amounting evidence suggests nucleosome organization is key for higher levels of chromatin packaging. However, studying it in higher eukaryotes is challenging due to the difficulty to manipulate nucleosomes in vivo and reconstitute their large higher-order chromatin domains in vitro. Facilitates chromatin transcription (FACT) is a H2A/B histone chaperone that displaces and re-assembles histones, being important for processes such as transcription. Recent studies report a minor role of FACT in chromatin architecture. Here, we studied chromatin organization upon rapid FACT degradation employing a multidimensional 3C technique with base-pair resolution (MCC-u) and genomic approaches. FACT loss leads to nucleosome depletion around active promoters, nano-scale domain collapse and emergence of larger nucleosome-free regions with increased protein binding. At a larger scale, we detect increased interactions between promoters across topologically associated domains. Thus, we demonstrate FACT plays a major role in chromatin organization and gain insights into the role of nucleosomes in genome architecture.

2026-03-30 | GSE314917 | GEO

FACT maintains nucleosomes around promoters and opposes to the spreading of chromatin factors, thereby playing a major role in chromatin architecture [MCC]

2026-03-30 | GSE314898 | GEO

FACT maintains nucleosomes around promoters and opposes to the spreading of chromatin factors, thereby playing a major role in chromatin architecture [ATAC-seq]

2026-03-30 | GSE314892 | GEO

FACT maintains nucleosomes around promoters and opposes to the spreading of chromatin factors, thereby playing a major role in chromatin architecture [ChIP-seq]

2026-03-30 | GSE314899 | GEO

FACT maintains nucleosomes around promoters and opposes to the spreading of chromatin factors, thereby playing a major role in chromatin architecture [CUT&TAG]

2026-03-30 | GSE314913 | GEO

FACT maintains nucleosomes around promoters and opposes to the spreading of chromatin factors, thereby playing a major role in chromatin architecture [RNA-Seq]

2026-03-30 | GSE314894 | GEO

FACT depletion demonstrates a role for nucleosome organization in TAD formation [1]

Project description:Mammalian genomes are organized into distinct chromatin structures, which include small-scale nucleosome arrays and large-scale topologically associating domains (TADs). The mechanistic interplay between chromatin structures across scales is poorly understood. Here, we investigate how changes in nucleosome architecture impact TAD organization by studying the role of the histone chaperone facilitates chromatin transcription (FACT) in 3D genome organization. We show that FACT depletion perturbs TADs, causing decreased insulation and weaker CTCF loops. These changes in TAD structure cannot be attributed to changes in chromatin occupancy of CTCF or cohesin and occur specifically in transcribed regions of the genome where we observe perturbed nucleosome organization in absence of FACT. FACT depletion therefore allows us to separate the role of CTCF binding from nucleosome architecture and to demonstrate that the organization of nucleosomes at TAD boundaries directly contributes to TAD formation.

2025-08-29 | GSE284532 | GEO

FACT depletion demonstrates a role for nucleosome organization in TAD formation [2]

2025-08-29 | GSE284529 | GEO

Abo1, a conserved bromodomain AAA-ATPase maintains global nucleosome occupancy and organization

Project description:Maintenance of the correct level and organization of nucleosomes is crucial for genome function. Here we uncover a role for a conserved bromodomain AAA-ATPase, Abo1, in maintenance of nucleosome architecture in fission yeast. Cells lacking abo1+ experience both a reduction and mis-positioning of nucleosomes at transcribed sequences in addition to increased intragenic transcription, phenotypes that are hallmarks of defective chromatin re-establishment behind RNA polymerase II. Abo1 is recruited to gene sequences and associates with histone H3 and the histone chaperone FACT. Furthermore, the distribution of Abo1 on chromatin is disturbed by impaired FACT function. The role of Abo1 extends to some promoters and also to silent heterochromatin. Abo1 is recruited to pericentromeric heterochromatin independently of the HP1 ortholog, Swi6, where it enforces proper nucleosome occupancy. Consequently, loss of Abo1 alleviates silencing and causes elevated chromosome mis-segregation. We suggest that Abo1 provides a histone chaperone function that maintains nucleosome architecture genome-wide.

2015-10-30 | GSE67410 | GEO

SCC1 interaction proteomics in Saccharomyces cerevisiae

Project description:FACT mediates cohesin function on chromatin Cohesin is a key regulator of genome architecture with roles in sister chromatid cohesion and the organisation of higher-order structures during interphase and mitosis. The recruitment and mobility of cohesin complexes on DNA are restricted by nucleosomes. Here we show that cohesin role in chromosome organization requires the histone chaperone FACT. Depletion of FACT in metaphase cells affects cohesin stability on chromatin reducing its accumulation at pericentric regions and binding on chromosome arms. Using Hi-C, we show that cohesin-dependent TAD (Topological Associated Domains)-like structures in G1 and metaphase chromosomes are disrupted in the absence of FACT. Surprisingly, sister chromatid cohesion is intact in FACT-depleted cells, although chromosome segregation failure is observed. Our results uncover a role for FACT in genome organisation by facilitating cohesin dependent compartmentalization of chromosomes into loop domains.

2019-10-03 | PXD014896 | Pride

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