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Spatial whole transcriptome analysis (GeoMx; Nanostring) of cervical lymph nodes (cLNs) from C57BL/6 mice infected intradermally (i.d.) with Mycobacterium tuberculosis (Mtb) reveals transcriptional changes at the edge of lesions.

ABSTRACT: Here, we used a mouse model of latent lymphatic M. tuberculosis (Mtb) infection (LTBI) to dissect the immunological mechanisms underlying LTBI containment versus reactivation. We show that immunosuppression-mediated reactivation of lymphatic LTBI and the subsequent induction of progressive disease can be prevented by vaccination with BCG or recombinant BCG (BCG::ESAT-6-PE25SS (abbreviated to PE25) even in the absence of CD4+ T cells. Spatial transcriptomics, network analysis and bioinformatic reveal that anti-CD4-mediated immunosuppression is associated with and enhanced immune response at the edge of TB lesions within the infection-draining cervical lymph nodes.

ORGANISM(S): Mus musculus

PROVIDER: GSE314979 | GEO | 2026/02/11

REPOSITORIES: GEO

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