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Developmental stage-specific gene expression in the two-spotted spider mite (Tetranychus urticae)

ABSTRACT: We generated 77-bp Illumina reads from single messenger RNA libraries from four diverse developmental stages of the two-spotted spider mite to maximally capture the complement of transcribed sequences across development. Adult, nymphal, larvae and embryonic stages were separated using sieves of various pore sizes, and mites of various developmental stages were carefully selected for transcriptome library preparation. Samples were a mix of males and females to capture male and female patterns of transcription, and were reared on beans (Phaseolus vulgaris cv California Red Kidney). The RNA-Seq data was used for validation of gene models predicted by EuGene, and to study patterns of gene expression across development.

ORGANISM(S): Tetranychus urticae

PROVIDER: GSE31527 | GEO | 2011/11/23

SECONDARY ACCESSION(S): PRJNA154989

REPOSITORIES: GEO

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Developmental stage-specific gene expression in the two-spotted spider mite (Tetranychus urticae)

Project description:We generated 77-bp Illumina reads from single messenger RNA libraries from four diverse developmental stages of the two-spotted spider mite to maximally capture the complement of transcribed sequences across development. Adult, nymphal, larvae and embryonic stages were separated using sieves of various pore sizes, and mites of various developmental stages were carefully selected for transcriptome library preparation. Samples were a mix of males and females to capture male and female patterns of transcription, and were reared on beans (Phaseolus vulgaris cv California Red Kidney). The RNA-Seq data was used for validation of gene models predicted by EuGene, and to study patterns of gene expression across development. Gene expression for spider mites from adult, nymph, larvae and embryonic developmental stages was examined (technical replicates were generated).

2011-11-23 | E-GEOD-31527 | biostudies-arrayexpress

Developmental stage-specific small RNA composition in the two-spotted spider mite (Tetranychus urticae)

Project description:We generated 38-bp Illumina reads from single messenger RNA libraries from three diverse developmental stages of the two-spotted spider mite to capture small RNA diversity across development. Adult, nymphal+larvae and embryonic stages were separated using sieves of various pore sizes, and mites of various developmental stages were carefully selected for small RNA library preparation. Samples were a mix of males and females to capture male and female patterns of small RNA composition and were reared on beans (Phaseolus vulgaris cv California Red Kidney). Small RNA reads were used for miRNA prediction, piRNA discovery, and for quantitation of small RNA-generating loci (i.e. expression across development).

2011-11-23 | GSE32005 | GEO

Developmental stage-specific small RNA composition in the two-spotted spider mite (Tetranychus urticae)

2011-11-23 | E-GEOD-32005 | biostudies-arrayexpress

Cyflumetofen resistance in the two-spotted spider mite Tetranychus urticae

Project description:Cyflumetofen is a novel acaricide on the international market with an unknown mode of action. Under laboratory conditions, we selected for high levels of cyflumetofen resistance in a susceptible mite strain and performed genome-wide gene-expression analysis. Differential expression between the resistant and susceptible strain was identified to study on the molecular level how mites develop resistance to this novel acaricide. Our results show that in T. urticae, the selection for cyflumetofen resistance resulted in a differential expression in only a limited number of genes. This transcriptomic study provides an unbiased look how cyflumetofen resistance triggers selection on the transcriptional level in T. urticae.

2017-05-12 | GSE62916 | GEO

Cyenopyrafen resistance in the two-spotted spider mite Tetranychus urticae

Project description:Cyenopyrafen is a novel acaricide with a currently unknown mode of action. We selected for high levels of cyenopyrafen resistance in a susceptible spider mite strain and previously published patterns of cross-resistance to other well-described acaricides (Khalighi et al., 2014). In addition, we also included an independent cyenopyrafen-susceptible mite strain (referred to as Akita) in this study. We performed transcriptomic analysis to identify on the molecular level how T. urticae develops resistance to this novel acaricide. Using this dataset, we show that the selection for cyenopyrafen resistance resulted in massive transcriptomic responses in T. urticae. The multi-gene family of cytochrome P450 monooxygenases (CYPs) exhibited a drastic differential expression with a significant transcriptional induction in the acaricide resistant strain. This transcriptomic study highlights the potential importance of CYPs in mite resistance to the cyenopyrafen acaricide.

2016-04-15 | GSE62915 | GEO

Identification of Wolbachia-responsive microRNAs in the two-spotted spider mite, Tetranychus urticae.

Project description: Not available

| S-EPMC4378230 | biostudies-literature

Cyflumetofen resistance in the two-spotted spider mite Tetranychus urticae

2017-05-12 | E-GEOD-62916 | biostudies-arrayexpress

Stably expressed housekeeping genes across developmental stages in the two-spotted spider mite, Tetranychus urticae.

Project description: Not available

| S-EPMC4379063 | biostudies-literature

Protocols for the delivery of small molecules to the two-spotted spider mite, Tetranychus urticae.

Project description: Not available

| S-EPMC5501582 | biostudies-literature

spatially- and tempospatially-stressed spider mites (Tetranychus urticae) vs control spider mites (Tetranychus urticae)

Project description:In this study, we profiled the transcriptional changes in a polyphagous spider mite, Tetranychus urticae, after adaptation to spatial and tempospatial stress. We show heritable down-regulation of genes encoding for core enzymes involved in the citric acid and gluconeogenesis/glycolyse pathways (glucose 6-phosphatase among others). Additionally, we observe heritable transcriptional changes in amino acid pathways (methionine, tyrosine and phenylalanine) and in laterally acquired genes from bacteria (cobalamin-independent methionine synthase). By similiar study results in other organisms, we argue that these heritable transcriptional changes (partially) underpin the changed life history traits observed in our experimental evolution set-up.

2016-05-02 | GSE55623 | GEO

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