ScRNAseq analysis of rat dorsal root ganglions from different peripheral injury treatment; a nerve transection, a crush 2 weeks after the cut and a resection of the neuroma 2 weeks afrer the initial cut

ABSTRACT: Peripheral nerve injury, whether caused by a cut, crush, or excessive stretch, can result in the formation of a painful neuroma at the site of damage. After a traumatic injury, regenerating nerves may develop a painful swelling at the severed end. This development is often accompanied by significant pain along the affected nerve's distribution, which can, in some cases, be more debilitating than the loss of motor function or sensation. It has been hypothesized that a proximal nerve crush may offer therapeutic benefits for painful neuromas by altering the axonal growth pattern and disrupting retrograde signaling. Despite existing treatment approaches, success in managing neuromas has been limited. Therefore, it is crucial to deepen our understanding of painful neuromas and develop more effective therapies. The molecular mechanisms underlying the formation and pain associated with symptomatic neuromas remain poorly understood. In this study, we thoroughly characterize the transcriptional changes in the dorsal root ganglion at single-cell resolution following proximal nerve crush versus nerve resection after neuronal transection.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE315353 | GEO | 2026/10/30

REPOSITORIES: GEO