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Characterization of the effect of hepatocyte nuclear factor 4 (Hnf4) silencing in enterocytes on gene expression in the developing Drosophila larval midgut

ABSTRACT: The main objective of this study was to characterize the effects of Hnf4 silencing in enterocytes (ECs) on the Drosophila midgut during larval development. Larval midguts from control animals and animals expressing Hnf4 RNAi in ECs were collected on day three and four after egg laying, corresponding to the late L2 and early L3 stages, respectively. Midguts were then processed for RNA extraction and mRNA sequencing. Our mRNA-seq study reveals significant changes in the transcriptome of control midguts between days three and four of larval development, with increased expression of numerous genes involved in lipid metabolism. Silencing Hnf4 largely suppresses this transcriptional switch, highlighting the role of this nuclear receptor in inducing intestinal lipid metabolism during larval development.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE315710 | GEO | 2026/03/07

REPOSITORIES: GEO

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