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RNA-seq profiling of METTL1 knockdown in human intestinal epithelial FHC cells

ABSTRACT: METTL1 is a key methyltransferase responsible for N7-methylguanosine (m7G) modification of RNA and has been implicated in multiple cellular processes. However, its role in human intestinal epithelial cells remains incompletely understood. In this study, we performed RNA sequencing (RNA-seq) to characterize transcriptomic changes induced by METTL1 knockdown in human fetal intestinal epithelial (FHC) cells. Stable METTL1 knockdown was achieved using lentiviral short hairpin RNA (shRNA), while control cells were infected with a non-targeting control shRNA lentivirus (sh-Ctrl). Knockdown efficiency was validated prior to sequencing. Total RNA was extracted from METTL1-knockdown and sh-Ctrl FHC cells, with three independent biological replicates per group. This dataset provides a transcriptomic resource for investigating the regulatory role of METTL1 in intestinal epithelial cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE315761 | GEO | 2026/01/12

REPOSITORIES: GEO

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METTL1 promotes hepatocarcinogenesis via m7G tRNA modification dependent translation control

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