Single‑cell RNA sequencing of orthotopic GBM tumors following oncolytic adenovirus treatment

ABSTRACT: Mice bearing intracranial GL261‑CAR tumors were treated intratumorally with PBS (control), oAd, or oAd‑mCD47/PD‑L1‑mIL‑12 (1 × 109 pfu). Tumors were harvested three days after the second viral injection and dissociated into single‑cell suspensions using the SeekMate Tissue Dissociation Reagent Kit A Pro (SeekGene, K01801‑30).Single‑cell RNA‑seq libraries were constructed using the SeekOne Digital Droplet Single‑Cell 3′ Library Preparation Kit (SeekGene, #K00202).Single‑cell transcriptomic data demonstrate that oAd‑mCD47/PDL1‑mIL‑12 orchestrates a multi‑faceted immune response: expanding cytotoxic and memory T‑cell subsets, polarizing macrophages toward an antigen‑presenting and chemokine‑secreting phenotype, and enhancing reciprocal signaling between immune and cancer cells to promote tumor clearance.

ORGANISM(S): Mus musculus

PROVIDER: GSE316315 | GEO | 2026/01/20

REPOSITORIES: GEO