Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Immune cell kinetics during Th17 adoptive trasfer-mediated experimental autoimmune encephalomyelitis

ABSTRACT: This study sought to characterive the spatial and temporal differences in comprehensive mononuclear immune cells throughout the disease course in a preclinical mouse model of central nervous system (CNS) autoimmune demyelinating disease. This dataset allows for the identification of transcriptionally distinct immune cell subsests within more general populations, inlcuding monocytes, neutrophils, microglia, T cells, and B cells, that are present in the blood and spinal cord during disease.

ORGANISM(S): Mus musculus

PROVIDER: GSE316626 | GEO | 2026/05/15

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptomic profiling of choroid plexus in experimental autoimmune encephalomyelitis

Project description:Multiple sclerosis (MS) is a demyelinating disease causing major neurological disability in young adults. We characterized the transcriptome of the choroid plexus (CP), which is part of the blood-brain barriers and the major site of cerebrospinal fluid (CSF) production, in the experimental autoimmune encephalomyelitis mouse model of MS. Genes encoding for adhesion molecules, chemokines and cytokines displayed the most altered expression, supporting the CP as a site of immune-brain interaction in MS. The gene encoding for lipocalin 2 (LCN2) was the most up-regulated, and CSF LCN2 levels coincided with the phases of active disease. Total RNA was isolated with Trizol (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s instructions. After quality assessment using the Agilent Bioanalyzer (Agilent Technologies, CA, USA), 100ng of total RNA from 3 pooled controls and 3 or 2 pooled samples of each experimental autoimmune encephalomyelitis (EAE) phase were amplified and labelled with the Illumina TotalPrep RNA Amplification Kit (Illumina Inc., San Diego, CA, USA). The labeled cRNA was then hybridized using the recommended protocol in a total of two Illumina Whole-Genome MouseRef-8 expression Beadchips (Illumina Inc., San Diego, CA, USA).

2012-06-29 | E-GEOD-35363 | biostudies-arrayexpress

Differential expression of genes in spinal cord folowing experimental autoimmune encephalomyelitis (EAE) in mice.

Project description:Microarrays were used to identify genes that were differently expressed in mouse spinal cord as a resut of experimental autoimmune encephalomyelitis (EAE), which is a model for demyelinating disease. Mice were injected with PLP peptide or vechicle.

2013-08-01 | E-GEOD-44989 | biostudies-arrayexpress

Differential expression of genes in spinal cord folowing experimental autoimmune encephalomyelitis (EAE) in mice.

2013-08-01 | GSE44989 | GEO