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Grape skin transcriptome in the berries grown on the vine treated with exogenous abscisic acid

ABSTRACT: The experiment was conducted using grapevines of Cabernet Sauvignon grown in a field. At veraison, the clusters in the vines were sprayed with a 400 mgl-1(+)-abscisic acid (ABA) solution. The ABA treated berries and non-treated berries (control) were harvested at 14 and 28 days after veraison and peeled. PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Kazuya Koyama. The equivalent experiment is VV16 at PLEXdb.

ORGANISM(S): Vitis vinifera

PROVIDER: GSE31664 | GEO | 2011/08/27

SECONDARY ACCESSION(S): PRJNA145397

REPOSITORIES: GEO

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Grape skin transcriptome in the berries grown on the vine treated with exogenous abscisic acid

Project description:The experiment was conducted using grapevines of Cabernet Sauvignon grown in a field. At veraison, the clusters in the vines were sprayed with a 400 mgl-1(+)-abscisic acid (ABA) solution. The ABA treated berries and non-treated berries (control) were harvested at 14 and 28 days after veraison and peeled. PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Kazuya Koyama. The equivalent experiment is VV16 at PLEXdb. ABA treated - developmental stage name: 14 days after veraison(3-replications); ABA treated - developmental stage name: 28 days after veraison(3-replications); untreated (control) - developmental stage name: 14 days after veraison(3-replications); untreated (control) - developmental stage name: 28 days after veraison(3-replications)

2011-08-26 | E-GEOD-31664 | biostudies-arrayexpress

High temperature effect on Cabernet Sauvignon berries

2011-08-26 | GSE31675 | GEO

Grape skin transcriptome in the berries cultured in vitro treated with exogenous abscisic acid

2011-08-26 | GSE31662 | GEO

High temperature effect on Cabernet Sauvignon berries

Project description:The experiment was conducted using four 11-year-old potted grapevines of V. vinifera L. cv. Cabernet Sauvignon grafted on SO4 (Selection Oppenheim No. 4) grown in a phytotron. Vines were trained on a Guyot trellising system and each vine carried 4-10 clusters of grapes. The experiment started approximately 1 week before veraison, when berry softening started, and continued to fruit maturity. The two temperature regimes consisted of a high day (06.00–20.00 h) temperature (max.35C) and a control (max. 25C). Under both conditions, the night-time (20.00–06.00 h) temperature was 20C. PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Kentaro Mori. The equivalent experiment is VV9 at PLEXdb developmental stage: 2 weeks - temperature: High (2-replications); developmental stage: 2 weeks - temperature: Control(2-replications); developmental stage: 4 weeks - temperature: High (2-replications); developmental stage: 4 weeks - temperature: Control(2-replications); developmental stage: 6 weeks - temperature: High (2-replications); developmental stage: 6 weeks - temperature: Control(2-replications)

2011-08-25 | E-GEOD-31675 | biostudies-arrayexpress

Gene expression associated with compatible viral diseases in berry

Project description:To understand the fruit changes and mechanisms involved in the compatible grapevine-virus interaction, we analyzed the berry transcriptome in two stages of development (veraison and ripening) in the red wine cultivar Cabernet Sauvignon infected with Grapevine leaf-roll-associated virus-3 (GLRaV-3). Analysis of global gene expression patterns indicate incomplete berry maturation in infected berries as compared to uninfected fruit suggesting viral infection interrupts the normal berry maturation process. PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Andrea Vega. The equivalent experiment is VV28 at PLEXdb.

2011-08-26 | GSE31660 | GEO

Gene expression associated with compatible viral diseases in berry

2011-08-25 | E-GEOD-31660 | biostudies-arrayexpress

Grape skin transcriptome in the berries cultured in vitro treated with exogenous abscisic acid

Project description:The softened green berries of Cabernet Sauvignon were cultured in a Petri dish. 0.3M sucrose solution with (±)-abscisic acid (ABA) and without (±)-ABA (control) were applied to the Petri dishes. The berry skins were sampled at 3 and 10 days after the application. PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Kazuya Koyama. The equivalent experiment is VV17 at PLEXdb. ABA treated - time: 3 days(2-replications); ABA treated - time: 10 days(2-replications); Non-treated (control) - time: 3 days(2-replications); Non-treated (control) - time: 10 days(2-replications)

2011-08-25 | E-GEOD-31662 | biostudies-arrayexpress

Long-term Salt & Water Stress in Grapes

Project description:Potted Cabernet Sauvignon vines in the greenhouse were exposed to irrigated controls, non-irrigated water-deficits, and saline treatments for 16 days. Plant shoot tips were harvested every 4 days (0,4,8,12, and 16 days) to measure the progression of changes of global gene expression due to the stress. PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Grant Cramer. The equivalent experiment is VV2 at PLEXdb.

2011-08-26 | GSE31677 | GEO

Integrated omics analysis reveals the norisoprenoid responses in Vitis vinifera L. cv. Cabernet Sauvignon berries to abscisic acid and synthetic auxin

Project description:The abscisic acid (ABA) increase and auxin decline are both indicators of ripening initiation in grape berry, and norisoprenoid accumulation also start at around the onset of ripening. To investigate the transcriptional and posttranscriptional regulation of the ABA and synthetic auxin 1-naphthaleneacetic acid (NAA) on norisoprenoid production, we performed time series GC-MS and RNA-seq analyses on Cabernet Sauvignon grape berries from pre-veraison to ripening. Higher levels of free norisoprenoids were found in ABA-treated mature berries in two consecutive seasons, and both free and total norisoprenoids were significantly increased by NAA in one season. The expression pattern of known norisoprenoid-associated genes in all samples and the up-regulation of specific alternative splicing isoforms of VviDXS and VviCRTISO in NAA-treated berries were predicted to contribute to it. Combined weighted gene co-expression network analysis (WGCNA) and promoter motif prediction analysis suggested that GATA26 and GATA28 could be potential regulators of norisoprenoid accumulation. Finally, the network analysis uncovered the interaction between previously identified switch genes, hormone-related genes and norisoprenoid-associated genes.

2020-05-12 | GSE150343 | GEO

Transcriptome Comparison of Cabernet Sauvignon Grape Berries from Two Regions with Distinct Climate

Project description:To obtain an interpretation from the view of transcriptome on distinct metabolite accumulation between ecologically different regions in China, next-generation sequencing technology was performed on E-L 31, 35 and 38 stages of Cabernet Sauvignon grape berries from Changli (CL, eastern) and Gaotai (GT, western). The transcript abundance of epoxycarotenoid dioxygenase and xanthoxin dehydrogenase required for ABA biosynthesis was significantly higher in the GT berries at E-L 35 and 38 stages compared with the CL berries, which may explain the relatively short maturation period of berries in the western region. Some genes required for carbohydrate metabolism, such as hexose transporter, L-idonate dehydrogenase and phosphoenolpyruvate carboxylase, were significantly up-regulated in the CL berries in relative to the GT berries, which positively correlated with the sugar and organic acid accumulations. Pathway enrichment analysis of differentially expressed genes revealed that the CL berries had higher levels of phenylpropanoid biosynthesis at E-L 38 stage than the GT berries, which may relate to the quick fading of the GT wines because of weak co-pigmentation. cDNA libraries generated from three developmental stages (E-L 31, 35 and 38) of Cabernet Sauvignon grape berries from Changli (CL, eastern) and Gaotai (GT, western) in China were sequenced using Illumina HiSeq 2000.

2014-11-21 | E-GEOD-63512 | biostudies-arrayexpress

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