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Differential gene expression profile of y79 retinoblastoma cells in response to Anti - HMGA2 siRNA treatment

ABSTRACT: The differential gene expression study was conducted in the Anti - HMGA2 siRNA treated Retinoblastoma cell (Y79). The transient transfection in Y79 cells were performed using Anti-HMGA2 siRNA . The concentration and the time of incubation for the transfection was optimised to increase the silencing efficiency and reduce the off -target effects. After 48 hours of transfection the cells were collected and the whole genome cDNA microarray was performed to interpret the differential gene expression regulated by HMGA2 in Y79 cells. The results of the microarray data has been validated.

ORGANISM(S): Homo sapiens

PROVIDER: GSE31687 | GEO | 2012/08/01

SECONDARY ACCESSION(S): PRJNA145653

REPOSITORIES: GEO

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Differential gene expression profile of y79 retinoblastoma cells in response to Anti - HMGA2 siRNA treatment

Project description:The differential gene expression study was conducted in the Anti - HMGA2 siRNA treated Retinoblastoma cell (Y79). The transient transfection in Y79 cells were performed using Anti-HMGA2 siRNA . The concentration and the time of incubation for the transfection was optimised to increase the silencing efficiency and reduce the off -target effects. After 48 hours of transfection the cells were collected and the whole genome cDNA microarray was performed to interpret the differential gene expression regulated by HMGA2 in Y79 cells. The results of the microarray data has been validated. One-color experiment,Organism: Human , Custom Whole Genome Human 8x60k designed by Genotypic Technology Pvt. Ltd. (Agilent-027114), Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442)

2012-07-31 | E-GEOD-31687 | biostudies-arrayexpress

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Differential expression of miRNAs in Retinoblastoma cells (Y79) in response to anti-HMGA2 siRNA treatment

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Project description:The Y79 retinoblastoma cells were exposed to oxidative stress conditions with hydrogen peroxide exposure. The differential gene expression profile on this condition was evaluated by comparing with untreated control Y79 cells. The cellular responses based on the differential gene expression was studied.

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Gene expression profiling of retinoblastoma Y79 cell line post EpCAM silencing using siRNA

Project description:Retinoblastoma Y79 cell line was treated with specific siRNA to silence EpCAM gene expression in vitro and RNA was isolated for microarray experiment to study the changes in the whole gene expression profiling in the Y79 cell line. The study identified 465 up-regulated genes (>1.0 fold) and 205 down-regulated genes (<0.5 fold) in response to knockdown of Ep-CAM.

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Whole genome microarray of T lymphoma invasion and metastasis (Tiam1) silenced retinoblastoma Y79 cells

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Project description:The proteins on the surface of Y79 retinoblastoma cell line were analyzed by cell surface biotinylation and streptavidin affinity purification.

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Y79 retinoblastoma cells: control vs. curcumin treated

Project description:miRNA profiling of curcumin treated Y79 cells with untreated Y79 cells (control). Aim of the study to see whether any oncogenes or tumor suppressor genes are regulated on curcumin treatment in Y79 cells. Agilent one-color experiment,Organism: Human ,Agilent-019118 Human miRNA Microarray 2.0 G4470B , Labeling kit: Agilent miRNA labeling reagent and Hybridization Kit Cat # 5190-0408

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