Microbiome analysis of control, radiation enteritis model, and treatment groups in rat stool samples using 16S rRNA sequencing

ABSTRACT: This study investigates the gut microbiome composition and diversity in three groups of rats: control, radiation enteritis model, and treatment (TG) groups. Total DNA was extracted from stool samples, PCR-amplified targeting 16S rRNA gene variable regions, and sequenced using Illumina MiSeq or NovaSeq platforms. Downstream bioinformatics analyses included sequence quality control, denoising (DADA2/OTU clustering), taxonomic classification, alpha and beta diversity evaluation, differential species abundance analysis, and functional prediction. The processed data include ASV/OTU tables, taxonomy assignments, and sample metadata.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE317110 | GEO | 2026/01/26

REPOSITORIES: GEO