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Single-cell transcriptomic profiling of NRasG12V/myrAKT-driven hepatocellular carcinoma with Sepp1 knockdown and selenium supplementation

ABSTRACT: The immune microenvironment of hepatocellular carcinoma (HCC) is strongly influenced by tumor-infiltrating neutrophils, whose functional heterogeneity and regulatory mechanisms remain incompletely defined. Selenium metabolism, mediated by selenoprotein P (Sepp1), plays an important role in redox homeostasis and immune regulation in the liver.

In this study, Ras/AKT-driven murine HCC models with Sepp1 knockdown were generated under control or selenium-supplemented conditions. Single-cell RNA sequencing was performed using the 10x Genomics Chromium platform to profile tumor tissues and the associated tumor microenvironment, with a particular focus on neutrophil populations.

This dataset enables systematic analysis of neutrophil transcriptional states associated with selenium metabolism and immunoregulatory pathways in HCC. The data provide a resource for investigating how alterations in Sepp1 expression and selenium availability shape tumor-immune interactions at single-cell resolution.

ORGANISM(S): Mus musculus

PROVIDER: GSE317333 | GEO | 2026/03/22

REPOSITORIES: GEO

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