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Gene expression in response to short and long term cAMP stimulation in the INS-1 insulinoma cell line

ABSTRACT: Long term exposure to incretin hormones is known to have salutory effects on beta cell function and viability. While short-term cAMP induction is known to have a signature CREB-CRTC target gene response, the long-term effects of cAMP on beta cell gene expression are less well understood. We used rat microarray analysis to compare the genome-wide gene expression response to short-term (2 hours) and long-term (16 hours) stimulations of the cAMP agonist forskolin in INS-1 insulinoma cells.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE31736 | GEO | 2011/11/22

SECONDARY ACCESSION(S): PRJNA145433

REPOSITORIES: GEO

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Gene expression in response to short and long term cAMP stimulation in the INS-1 insulinoma cell line

Project description:Long term exposure to incretin hormones is known to have salutory effects on beta cell function and viability. While short-term cAMP induction is known to have a signature CREB-CRTC target gene response, the long-term effects of cAMP on beta cell gene expression are less well understood. We used rat microarray analysis to compare the genome-wide gene expression response to short-term (2 hours) and long-term (16 hours) stimulations of the cAMP agonist forskolin in INS-1 insulinoma cells. INS-1 cells were exposed to forskolin for 2 or 16 hours in RPMI medium containing 10% serum. Control samples wer incubated for the same time without forskolin. Extracted RNA was used for hybridization on Affymetrix rat 1.0 st gene arrays.

2011-11-22 | E-GEOD-31736 | biostudies-arrayexpress

Transcription profiling of rat insulinoma line INS-1 to identify targets of HNF1b mutants in INS-1

Project description:A rat insulinoma cell line (INS-1) was generated that contains a FRT site for FLP recombinase mediated site specific integration of specific genes. In addition, the line contains the tetracycline repressor allowing tetracycline (tet) induction of the introduced gene. By comparing the gene expression profiles of uninduced and tet-induced cells, target genes of introduced transcription factors HNF1b and HNF1b mutants were identified. Two different clones containing the same transcription factor were analyzed in the non-induced and tet-induced (24 h) state.

2008-06-11 | E-GEOD-1590 | biostudies-arrayexpress

Rattus norvegicus

Project description:Gene expression in response to short and long term cAMP stimulation in the INS-1 insulinoma cell line

| PRJNA145433 | ENA

Transcription profiling of rat insulinoma INS-1 cell lines (FLP-In T-REx)

Project description:Four rat insulinoma cell lines (INS-1) were generated that contain a FRT site for FLP recombinase mediated site specific integration of specific genes. In addition, the cell lines contain the tetracycline repressor allowing tetracycline (tet) induction of the introduced gene. By comparing the gene expression profiles of uninduced and tet-induced cells, target genes of introduced transcription factors or other signal tranduction molecules can be identified. The expression profiles of the parental INS-1 cell line and the four derivatives were determined on a RAE230A array, showing that the highly differentiated phenotype of the parental cell line was maintained in the clones.

2008-06-11 | E-GEOD-1591 | biostudies-arrayexpress

Gene expression changes in response to doxycyline-induced expression of proinsulin (C96Y)-GFP in an INS-1 insulinoma cell line

Project description:A doxycyline-inducible INS-1 insulinoma cell line expressing proinsulin (C96Y)-GFP was engineered. Addition of doxycyline causes the production of the proinsulin (C96Y)-GFP, which is retained in the endoplasmic reticulum. This study analyzes the gene expression changes that occur after doxycyline-induced expression of proinsulin (C96Y)-GFP for 24h, 48h and 5 days. Expression changes were compared between control un-induced cells and cells treated with doxycyline. Three replicates (experiments) were performed for each time point. Stable mutant insulin-expressing insulinoma cells were treated or not with doxycyline for 24h, 48h or 5 days and RNA was extracted for expression analysis.

2010-07-29 | E-GEOD-22537 | biostudies-arrayexpress

Gene expression changes in response to doxycyline-induced expression of proinsulin (C96Y)-GFP in an INS-1 insulinoma cell line

2010-06-24 | GSE22537 | GEO

Meg3 binding sites in mouse insulinoma cells

Project description:We have shown that increased β-cell proliferation in functioning pancreatic neuroendocrine tumors (insulinomas) correlated with reduced expression of the long non-coding RNA Meg3 and increased expression of the oncogenic receptor c-Met. To investigate the target binding sites of Meg3 in and around the c-Met gene, we did ChIRP-Seq using biotinylated probes from the mouse Meg3 RNA sequence. This would help us better understand how Meg3 regulates ithe expression of c-Met to control β-cell proliferation in insulinoma cells.

2017-09-20 | GSE99798 | GEO

Expression data from rat insulinoma INS-1 cells under glucotoxic condition

Project description:Expression data from rat insulinoma INS-1 cells under glucotoxic condition

| PRJNA454198 | ENA

Transcription profiling of mouse MIN6 insulinoma beta cells following knock-down of murine Pdx1

Project description:In order to identify genes that are regulated by Pdx1, MIN6 insulinoma cells were transduced with adenovirus expressing a short hairpin RNA directed against murine Pdx1 (AdshPdx1) or luciferase (AdshLuc) or not transduced at all (untreated). RNA was harvested and reverse transcribed. Reduced expression of Pdx1 and established Pdx1 targets was confirmed. The RNA was then submitted for labeling and hybridization to the PancChip cDNA microarray.

2010-01-01 | E-MTAB-132 | biostudies-arrayexpress

Time course treatment with Palmitate and thapsigargin of insulinoma INS-1E cells

Project description:Time course treatment with 0.4 mM Palmitate and 200 nM thapsigargin of insulinoma INS-1E cells. Timepoints; 0, 4, 16 and 24h in biological duplicates within an iTRAQ 8 set for palmitate and thapsigargin, respectively.

2020-08-18 | PXD020851 | JPOST Repository

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