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Transcriptional responses of normal human dermal fibroblasts (NHDF) induced by Borrelia burgdorferi sensu stricto

ABSTRACT: The aim of the study was to compare the global transcriptional responses elicited in NHDF cells by three different strains of Borrelia burgdorferi ss (the agent of Lyme borreliosis), representative of different stages in the life cycle of Borrelia: one reference strain isolated from a tick (strain N40), and two invasive strains isolated from skin biopsy of erythema migrans (strain Pbre c4) and acrodermatitis chronica atrophians skin lesions (strain 1408 c1).

ORGANISM(S): Homo sapiens

PROVIDER: GSE31740 | GEO | 2012/08/02

SECONDARY ACCESSION(S): PRJNA145441

REPOSITORIES: GEO

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Transcriptional responses of normal human dermal fibroblasts (NHDF) induced by Borrelia burgdorferi sensu stricto

Project description:The aim of the study was to compare the global transcriptional responses elicited in NHDF cells by three different strains of Borrelia burgdorferi ss (the agent of Lyme borreliosis), representative of different stages in the life cycle of Borrelia: one reference strain isolated from a tick (strain N40), and two invasive strains isolated from skin biopsy of erythema migrans (strain Pbre c4) and acrodermatitis chronica atrophians skin lesions (strain 1408 c1). Three different experimental conditions have been tested: (1) unstimulated NHDF vs NHDF stimulated by Borrelia strain N40 / (2) unstimulated NHDF vs NHDF stimulated by Borrelia strain Pbre c4 / (3)M-BM- unstimulated NHDF vs NHDF stimulated by Borrelia strain 1408 c1. There is 2 biological replicates for each condition. All NHDF stimulation have been performed in independent experiments.

2012-08-01 | E-GEOD-31740 | biostudies-arrayexpress

Identification of the differences between gene expression in normal human dermal fibroblasts (NHDF cell line) infected with Borrelia burgdorferi sensu lato spirochetes

Project description:The similarity of Lyme borreliosis to other diseases and the complex pathogenesis cause diagnostic and therapeutic difficulties. Changes at the cellular and molecular level after Borrelia sp. infection remain still poorly understood. Therefore, the present study focused on the gene expression in human dermal fibroblasts in differentiation of infection with Borrelia garinii, Borrelia afzelii and Borrelia burgdorferi sensu stricto spirochetes. For microarray analysis 10 samples were used: 3 control samples - K, 2 samples of NHDF cells infected with Borrelia garinii - G, 2 samples of NHDF cells infected with Borrelia afzelii - A and 3 samples of NHDF cells infected with Borrelia burgdorferi sensu stricto - SS.

2015-05-12 | E-GEOD-68741 | biostudies-arrayexpress

Proteomic analysis of three Borrelia burgdorferi sensu lato species and disseminating clones: relevance for Lyme vaccine design.

Project description:Lyme borreliosis is first characterized by a cutaneous inflammation, the erythema migrans, and if dissemination occurs after an infected tick bite cutaneous, nervous and articular manifestations appear. Although antibiotic treatments are efficient in the early stage of the infection, a significant number of patients develop disseminated manifestations due to unnoticed or absence of erythema migrans, or to inappropriate treatment. Vaccine could be an efficient approach to decrease Lyme disease incidence. We have developed a proteomic approach based on a Ge-LC-MS/MS strategy to identify new vaccine candidates. We analyzed a disseminating clone and the associated wild type strain for each major pathogenic Borrelia species: B. burgdorferi sensu stricto, B. garinii and B. afzelii. We managed to identify proteins specific and common to the disseminating clones of the 3 main species. In parallel, we used a spectral counting strategy in order to identify up-regulated proteins common to the clones. Thus, we identified 40 proteins that are potentially involved in bacterial virulence and could be of interest in the development of a new vaccine.

2015-02-16 | PXD000876 | Pride

Identification of the differences between gene expression in normal human dermal fibroblasts (NHDF cell line) infected with Borrelia burgdorferi sensu lato spirochetes

2015-05-12 | GSE68741 | GEO

NHDF Cells (Normal Human Dermal Fibroblasts): Control (unstimulated fibroblasts) vs. Borrelia infected cells

Project description:Transcriptional profiling of NHDF Cells comparing control untreated fibroblasts with fibroblasts coincubated with three different species of the Borrelia burgdorferi sensu lato group.

2016-05-22 | GSE77058 | GEO

Transcriptome Assessment of Erythema Migrans Skin Lesions

Project description:Transcriptome Assessment of Erythema Migrans Skin Lesions

| PRJNA647878 | ENA

Comprehensive transcriptome analysis regarding aging using normal human derived fibroblasts (NHDF)

Project description:We determined the gene expression changes by the cDNA status using NHDF cells at early passage and at later passage

2018-11-30 | GSE107483 | GEO

Genome-wide analysis of SHOX targets in NHDF cells expressing SHOX WT vs SHOX HM mutant

Project description:To elucidate the underlying molecular mechanisms and to better understand the broad phenotypic spectrum of SHOX deficiency, we aimed to identify novel SHOX targets. We analyzed differentially expressed genes in SHOX-overexpressing human fibroblasts (NHDF)

2021-06-01 | GSE169732 | GEO

Borrelia burgdorferi sensu lato

Project description:Borrelia burgdorferi species complex population genomics study with the focus on Borrelia garinii.

| PRJNA662729 | ENA

Transcriptional responses by human skin cells to high-voltage electric field: Sham vs. SE-14000 stimulation

Project description:Transcriptional profiling of SE-14000 stimulated wound healing associated cells (HaCat, NHDF, HMEC-1) comparing sham-treated cells. Goal was to determine the effects of SE-14000 in the human skin cells (HaCat, NHDF, HMEC-1).

2021-02-19 | GSE110830 | GEO

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