Dataset Information

Neofusicoccum laricinum disrupts larch defenses through coordinated cell wall degradation and antioxidant suppression

ABSTRACT: Larch (Larix spp.), a key species in China's reforestation efforts, faces increasing threats from shoot blight caused by Neofusicoccum laricinum. This study characterized the biological traits, virulence mechanisms, and host interactions of this pathogen to inform disease management. Twenty-five N. laricinum strains were isolated from six regions in Northeast China and identified through morphological and molecular analysis. Comprehensive growth assessments revealed optimal development at 18°C and pH 9-11, with significant strain-specific variation in virulence (lesions 18.4-38.8 mm). Pathogenicity assays revealed that the hypervirulent TM02 strain exhibited early and robust production of cell wall-degrading enzymes，such as pectin methylgalacturonase (PMG, 208.9 U/mg) and polygalacturonase (PG, 54.9 U/mg), correlated with its aggressive infection phenotype. Biochemical analyses revealed that the pathogen actively disrupted host oxidative defenses, with superoxide dismutase (SOD) activity peaking at 3 dpi (456.7 U/g·min-1) before decreasing to 0.8× control levels by 7 dpi, whereas peroxidase (POD) activity exhibited a transient 4.6-fold increase followed by rapid suppression. Transcriptome analysis revealed generally downregulation of defense genes, mainly cellulose synthase (21/25 genes) and peroxidase (38/45 genes), with –10.5-fold inhibition of Ces-g8671 and an –11.4-fold reduction in Pod-g18614 expression, indicating that pathogens can simultaneously damage larch cell wall synthesis and the ROS scavenging defense system. These findings establish N. laricinum’s sophisticated two-phase infection strategy: initial physical breach of cell walls facilitated by CWDEs, followed by systematic suppression of host antioxidant defenses. This study identifies specific molecular targets for developing intervention strategies and provides critical insights into host‒pathogen dynamics in larch plantations under climate change scenarios.

ORGANISM(S): Larix kaempferi

PROVIDER: GSE317540 | GEO | 2026/01/27

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other
	GSE317540_gene_fpkm_all_samples.txt.gz	Txt
	GSE317540_unique_transcripts.fasta.gz	Fasta.gz

Items per page:

1 - 3 of 3

Similar Datasets

Project description:In susceptible plant hosts, co-evolution has favoured viral strategies to evade host defenses and utilize resources to their own benefit. The degree of manipulation of host gene expression is dependent on host-virus specificity and certain abiotic factors. In order to gain insight into global transcriptomic changes for a geminivirus pathosystem, South African cassava mosaic virus [ZA:99] (SACMV-ZA:99]) and Arabidopsis thaliana, 4 x 44K Agilent microarrays were adopted. After normalization, a 2-fold change filtering of data (p<0.05) identified 1,820 differentially expressed genes in apical leaf tissue. A significant increase in differential gene expression over time (451 genes at 14 dpi, 742 genes at 24 dpi, and 1011 genes at 36 dpi) was observed. This increase in expression, correlated with an increase in SACMV accumulation as virus copies were 5-fold higher at 24 dpi and 6-fold higher at 36 dpi than at 14 dpi (1.1x104 virus copies present at 14 dpi, 5.7x104 copies at 24 dpi, and 6.3x104 copies at 36 dpi). Many 2-fold genes were primarily involved in stress and defense responses, phytohormone signalling pathways, cellular transport, cell-cycle regulation, transcription, oxidation-reduction, and other metabolic processes. Forty-one genes (2.3%) were shown to be continuously expressed across the infection period, indicating that the majority of genes were transient and unique to a particular time point. Plant signalling networks were disrupted and manipulated by SACMV-[ZA:99] in order to affect homeostasis and antagonize host’s defense responses. At the same time, an adaptive response was initiated to reprogramme metabolism and divert energy from growth-related processes to defense, all leading to disruption of normal biological host processes. Comparisons between SACMV-[ZA:99] with plant-infecting RNA and DNA viruses revealed similarities and differences in expression patterns among viruses, showing either general defense or virus-specific responses. Within the Geminiviridae family in particular, similarities in cell-cycle regulation and gene expression patterns correlated between SACMV-[ZA:99] and Cabbage leaf curl virus (CaLCuV) but differences were also evident. For instance, CaLCuV showed antagonistic interactions between Salicyclic Acid (SA) and Jasmonic Acid (JA) pathways, whereas SACMV displayed synergism. Differences in gene induction, repression and outcome between the two geminiviruses clearly demonstrated host-specific interactions with SACMV-[ZA:99] leading to infection. To our knowledge this is the first geminivirus study identifying differentially expressed transcripts across 3 time points

Project description:In susceptible plant hosts, co-evolution has favoured viral strategies to evade host defenses and utilize resources to their own benefit. The degree of manipulation of host gene expression is dependent on host-virus specificity and certain abiotic factors. In order to gain insight into global transcriptomic changes for a geminivirus pathosystem, South African cassava mosaic virus [ZA:99] (SACMV-ZA:99]) and Arabidopsis thaliana, 4 x 44K Agilent microarrays were adopted. After normalization, a 2-fold change filtering of data (p<0.05) identified 1,820 differentially expressed genes in apical leaf tissue. A significant increase in differential gene expression over time (451 genes at 14 dpi, 742 genes at 24 dpi, and 1011 genes at 36 dpi) was observed. This increase in expression, correlated with an increase in SACMV accumulation as virus copies were 5-fold higher at 24 dpi and 6-fold higher at 36 dpi than at 14 dpi (1.1x104 virus copies present at 14 dpi, 5.7x104 copies at 24 dpi, and 6.3x104 copies at 36 dpi). Many 2-fold genes were primarily involved in stress and defense responses, phytohormone signalling pathways, cellular transport, cell-cycle regulation, transcription, oxidation-reduction, and other metabolic processes. Forty-one genes (2.3%) were shown to be continuously expressed across the infection period, indicating that the majority of genes were transient and unique to a particular time point. Plant signalling networks were disrupted and manipulated by SACMV-[ZA:99] in order to affect homeostasis and antagonize hostM-bM-^@M-^Ys defense responses. At the same time, an adaptive response was initiated to reprogramme metabolism and divert energy from growth-related processes to defense, all leading to disruption of normal biological host processes. Comparisons between SACMV-[ZA:99] with plant-infecting RNA and DNA viruses revealed similarities and differences in expression patterns among viruses, showing either general defense or virus-specific responses. Within the Geminiviridae family in particular, similarities in cell-cycle regulation and gene expression patterns correlated between SACMV-[ZA:99] and Cabbage leaf curl virus (CaLCuV) but differences were also evident. For instance, CaLCuV showed antagonistic interactions between Salicyclic Acid (SA) and Jasmonic Acid (JA) pathways, whereas SACMV displayed synergism. Differences in gene induction, repression and outcome between the two geminiviruses clearly demonstrated host-specific interactions with SACMV-[ZA:99] leading to infection. To our knowledge this is the first geminivirus study identifying differentially expressed transcripts across 3 time points A three time-point (14, 24, and 36 dpi) study was carried out to identify differentially expressed genes in SACMV-[ZA:99] infected Arabidopsis leaf cells using a direct comparison design against mock-inoculated controls. Three biological replicates and 1 technical replicate for both SACMV-[ZA:99]-infected and mock-inoculated controls were conducted at each time point.

Dataset Information

Neofusicoccum laricinum disrupts larch defenses through coordinated cell wall degradation and antioxidant suppression

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets