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Knock-down of lncRNA SNHG26 in human embryonic stem cells

ABSTRACT: Development of long-read RNA sequencing technologies has paved the way to the exploration of RNA isoform diversity and its relevance in regulating cell fate. However, identifying new functional isoforms is still very difficult. Here, we leverage long-read RNA sequencing to study changes in isoforms during somatic cell reprogramming and identify novel isoforms occurring throughout cell state transitions. We demonstrate tight regulation of non-coding isoforms and show that isoform switching plays previously overlooked functional roles and outcomes in gene regulation and cell fate changes. We uncover a novel long non-coding RNA, Snhg26, that undergoes isoform switching during reprogramming to enhance the conversion of differentiated cells towards the pluripotent state. Here, we show the knockdown of the human SNHG26 is associated with loss of naive pluripotency markers in human embryonic stem cells. Together, our study provides a comprehensive resource to study full-length isoform regulation and mechanisms at stake during reprogramming, but more importantly it demonstrates the power of lrRNAseq to identify functionally relevant gene isoforms in the context of pluripotency.

ORGANISM(S): Homo sapiens

PROVIDER: GSE317557 | GEO | 2026/08/01

REPOSITORIES: GEO

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Long read RNA sequencing reveals a new long non-coding RNA that regulates somatic cell reprogramming and pluripotency

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