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Gene expression in the mouse embryonic small intestine in the presence or absence of E-cadherin

ABSTRACT: Gene expression was compared between E18.5 E-cadherin conditional knockout (cKO) small intestine and E18.5 control mouse small intestine.

ORGANISM(S): Mus musculus

PROVIDER: GSE31771 | GEO | 2012/02/28

SECONDARY ACCESSION(S): PRJNA145271

REPOSITORIES: GEO

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Gene expression in the mouse embryonic small intestine in the presence or absence of E-cadherin

Project description:Gene expression was compared between E18.5 E-cadherin conditional knockout (cKO) small intestine and E18.5 control mouse small intestine. E18.5 mouse small intestine was collected from control and E-cadherin conditional knockout mice. RNA was prepared. Affymetrix Mouse Gene 1.0 gene arrays were used to interrogate gene expression. Data was analyzed using dChip software.

2012-02-28 | E-GEOD-31771 | biostudies-arrayexpress

Gene expression in the mouse embryonic small intestinal epithelium in the presence or absence of GATA4 and GATA6

Project description:Gene expression was compared between E18.5 Gata4Gata6 double conditional knockout (cKO) small intestinal epithelium and E18.5 control mouse small intestinal epithleium. E18.5 mouse small intestine was collected from control and Gata4Gata6 double conditional knockout mice. Epithelial cells were isolated, and RNA was prepared. Affymetrix Mouse Gene 1.0 gene arrays were used to interrogate gene expression. Data was analyzed using dChip software.

2014-04-30 | E-GEOD-49471 | biostudies-arrayexpress

Gene expression in the mouse embryonic small intestinal epithelium in the presence or absence of GATA4 and GATA6

Project description:Gene expression was compared between E18.5 Gata4Gata6 double conditional knockout (cKO) small intestinal epithelium and E18.5 control mouse small intestinal epithleium.

2014-04-30 | GSE49471 | GEO

Effect of E-cadherin by N-cadherin replacement in mouse intestinal epithelial cells

Project description:We conditionally substituted E-cadherin (E-cad; cadherin 1) with N-cadherin (N-cad; cadherin 2) during intestine development by generating mice in which an Ncad cDNA was knocked into the Ecad locus We used microarray to follow gene expression changes derived from lack of E-cadherin on intestinal epithelial cells

2017-05-01 | GSE81920 | GEO

Transcriptional profiling of the developing murine intestine

Project description:To identify transcriptional changes during the development of the small intestine, we performed scRNAseq using MULTI-seq on 86,845 cells collected from embryos at E13.5-E18.5. We additionally identified transcriptional changes along the proximal-distal axis of the small intestine by isolating RNA from 2.5mm fragments of the tissue that had been cut along this axis. Mice were collected from Pdgfra-H2BGFP mice to enrich for mesenchymal populations.

2023-09-11 | GSE233407 | GEO

VE-cadherin associated proteins in the absence of lncRNA LASSIE

Project description:The shear stress-regulated lncRNA LASSIE interacts with junctional proteins (e.g. PECAM-1, which interacts with VE-cadherin) and influences endothelial barrier function. Here we characterize the remodeling of the VE-Cadherin complex by the lncRNA LASSIE. LASSIE silenced HUVECs were subjected to co-immunoprecipitation using an anti-VE-cadherin antibody. Differentially associated proteins were identified by Mass spectrometry. This analysis revealed a significantly decreased association of cytoskeleton-linked proteins with VE-cadherin after silencing of LASSIE. Functional assays confirmed this result and characterized LASSIE as a stabilizer of junctional complexes in endothelial cells, important for normal shear stress sensing and barrier function.

2021-09-09 | PXD018725 | Pride

Differential miRNA expression caused by Dicer knockout in small intestine

Project description:Dicer knockout cause lipid metabolism dysregulation in small intestine. Analyses of transcriptomics and proteomics reveal key enzymes affected by Dicer knockout. Conditional knockout of Dicer in adult mice

2012-01-07 | E-GEOD-34909 | biostudies-arrayexpress

Differential gene expression caused by Dicer knockout in small intestine

2012-01-07 | E-GEOD-34905 | biostudies-arrayexpress

Human small intestine

Project description:Human small intestine Metagenome

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Global transcriptional response of E. coli to the presence and absence of bile salts

Project description:Unlike many other types of diarrheagenic bacteria that act primarily in the small intestine, O157:H7 expresses virulence primarily in the large intestine. In this study, microarray analysis is employed to examine the transcriptional response of O157:H7 to bile treatment, to gain insight into how bile affects virulence and whether bile might be temporally defending the small intestine against virulence by these bacteria. Keywords: Expression profiling of two different growth conditions

2012-07-31 | GSE20380 | GEO

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