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Effect of PPARγ2 acetylation in at lysine 382 in mouse beige adipocyte differentiation

ABSTRACT: PPARr is a master regulator of adipogenesis which undergo different post translational modification during adipogenesis. This post translational modification of PPARγ control unique subset of genes rather full PPARr target genes. Recently we reported that SIRT7 deacetylate PPARr2 at lysine 382 residue and regulate lipogenesis in C3H10T1/2 cells. Here, we investigate this SIRT7-mediated PPARγ2 lysine deacetylation in mouse beige adipocyte differentiation of subcutaneous white AT (scWAT) stromal vascular fraction (SVF) cell line. At first we prepared PPARr2 K382 acetylation (KQ) and deacetylation (KR) mimicking mutant expressing mouse scWAT SVF cell line. After beige adipocyte differentiation, we isolated total RNA at day 2 and performed RNA-seq analysis.

ORGANISM(S): Mus musculus

PROVIDER: GSE317859 | GEO | 2026/06/18

REPOSITORIES: GEO

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