Transcriptomic analysis in relation to adventitious rooting of different petunia species
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ABSTRACT:
ORGANISM(S): Petunia axillaris Petunia integrifolia subsp. inflata
PROVIDER: GSE317986 | GEO | 2026/01/30
REPOSITORIES: GEO
Similar Datasets
Project description:In order to identify genes specifically induced during various developmental stages of Adventitious Root (AR) formation (described in Ahkami et al. 2009) in leafy cuttings of Petunia hybrida (line W115) and to describe the series of physiological processes during adventitious rooting, a microarray-based transcriptome analysis in the stem base of the cuttings was conducted. The microarray was described by Breuillin et al. (2010) and included a normalized cDNA library from different time points after taking the cuttings from mother plant. Because physiological processes and molecular changes specifically involved in AR formation were considered as of major interest, rather than those associated with wound responses, a filtration approach was chosen to eliminate primarily wound-responsive genes. This study is mainly focused on changes in transcript abundances of genes related to specific metabolic pathways or cellular events including primary metabolism, membrane transport, cell division or signalling during various phases of AR formation. The overall design of the custom microarray used in this study is described in Breuillin et al. (2010). In brief, a database of 24,816 unigene sequences (including 4,700 ESTs from P. hybrida cutting base and all public available P. hybrida and P. axillaris sequences) was used for construction of a custom microarray.
2014-05-17 | E-GEOD-57752 | biostudies-arrayexpress
Project description:In order to identify genes specifically induced during various developmental stages of Adventitious Root (AR) formation (described in Ahkami et al. 2009) in leafy cuttings of Petunia hybrida (line W115) and to describe the series of physiological processes during adventitious rooting, a microarray-based transcriptome analysis in the stem base of the cuttings was conducted. The microarray was described by Breuillin et al. (2010) and included a normalized cDNA library from different time points after taking the cuttings from mother plant. Because physiological processes and molecular changes specifically involved in AR formation were considered as of major interest, rather than those associated with wound responses, a filtration approach was chosen to eliminate primarily wound-responsive genes. This study is mainly focused on changes in transcript abundances of genes related to specific metabolic pathways or cellular events including primary metabolism, membrane transport, cell division or signalling during various phases of AR formation.
2014-05-17 | GSE57752 | GEO
Project description:Transcriptomic analysis in relation to adventitious rooting of different petunia species
| PRJNA1416273 | ENA
Project description:Arctic alpine species experience extended periods of cold and unpredictable conditions during flowering. Thus, often, alpine plants use both sexual and asexual means of reproduction to maximise fitness and ensure reproductive success. We used the arctic alpine perennial Arabis alpina to explore the role of prolonged cold exposure on adventitious rooting. We exposed plants to 4 °C for different durations and scored the presence of adventitious roots on the main stem and axillary branches. Our physiological studies demonstrated the presence of adventitious roots after 21 weeks at 4 °C saturating the effect of cold on this process. Notably, adventitious roots on the main stem developingin specific internodes allowed us to identify the gene regulatory network involved in the formation of adventitious roots in cold using transcriptomics. These data and histological studies indicated that adventitious roots in A. alpina stems initiate during cold exposure and emerge after plants experience growth promoting conditions. While the initiation of adventitious root was not associated with changes of DR5 auxin response and free endogenous auxin level in the stems, the emergence of the adventitious root primordia was. Using the transcriptomic data, we discerned the sequential hormone responses occurring in various stages of adventitious root formation and identified supplementary pathways putatively involved in adventitious root emergence, such as glucosinolate metabolism. Together, our results highlight the role of low temperature during clonal growth in alpine plants and provide insights on the molecular mechanisms involved at distinct stages of adventitious rooting.
2024-04-01 | GSE176054 | GEO
Project description:Our previous studies have shown that exogenous ethylene (ETH) may induce plant adventitious root development in cucumber. In this study, transcriptome technique was used to explore the key genes in ETH-induced rooting. The results revealed that ETH regulated 1415 diferentially expressed genes (DEGs) during rooting, among which 687 DEGs were up-regulated and 728 DEGs were down-regulated. According to Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis, the critical pathways involved in ETH-induced adventitious root development were selected for further study, including carbon metabolism [starch and sucrose metabolism, glycolysis / gluconeogenesis, citrate cycle (TCA cycle), oxidative phosphorylation, fatty acid biosynthesis and fatty acid degradation], secondary metabolite biosynthesis (phenylalanine metabolism and flavonoid biosynthesis) and plant hormone signal transduction. In carbon metabolism, ETH reduced t the expression of CsHK2, CsPK2 and CsCYP86A1, whereas enhanced the expression of CsBAM1 and CsBAM3. Moreover, ETH negatively regulated the transcript level of CsPAL and CsF3’M and positively mediated that of CsPAO in secondary metabolite biosynthesis pathway. Additionally, ETH could induce adventitious rooting by negatively regulating auxin and ETH signal transduction-related genes (CsLAX5, CsGH3.17, CsSUAR50 and CsERS) and positively regulating ABA and BR signaling transduction-related genes (CsPYL1, CsPYL5, CsPYL8, CsBAK1 and CsXTH3) . Furthermore, the results of real-time PCR about the mRNA levels of these genes were consistent with transcriptome results. Therefore, ETH may induce adventitious root development by regulating carbon metabolism-related genes, secondary metabolite biosynthesis-related genes and plant hormone signal transduction-related genes.
2021-11-30 | GSE189315 | GEO
Project description:Adventitious rooting (AR) is an obligatory step for vegetative propagation of commercial woody species. Paper industries have interest in Eucalyptus globulus Labill and its hybrids due to low lignin and lipid contents, which facilitate cellulose extraction. However, this species and some of its hybrids are recalcitrant to rooting, often requiring exogenous auxin supply. Here we performed a comparative analysis of proteome changes during AR of E. globulus and the easy-to-root species Eucalyptus grandis W. Hill and the effects of exogenous auxin in different phases of the process (induction and formation), using a label-free quantification method. We identified 398 differentially abundant proteins, which were predicted to be involved in different biological pathways, mainly oxidative stress, energy metabolism and photosynthesis. Notable differences between species included proteins involved in oxidative stress, carbon and secondary metabolism. Exogenous auxin appeared to affect the availability of carbon sources and other phytohormones, besides cell cycle and microtubule- related proteins. Important players were also identified in each phase of AR. This is the first in depth analysis of protein changes during AR in Eucalyptus. The findings can be used in future studies to evaluate rooting competence in different genotypes and provide leads for AR improvement.
2019-08-19 | PXD013924 | Pride
Project description:Adventitious rooting (AR) is an obligatory step for vegetative propagation of commercial woody species. Paper industries have interest in Eucalyptus globulus Labill and its hybrids due to low lignin and lipid contents, which facilitate cellulose extraction. However, this species and some of its hybrids are recalcitrant to rooting, often requiring exogenous auxin supply. Here we performed a comparative analysis of proteome changes during AR of E. globulus and the easy-to-root species Eucalyptus grandis W. Hill and the effects of exogenous auxin in different phases of the process (induction and formation), using a label-free quantification method. We identified 398 differentially abundant proteins, which were predicted to be involved in different biological pathways, mainly oxidative stress, energy metabolism and photosynthesis. Notable differences between species included proteins involved in oxidative stress, carbon and secondary metabolism. Exogenous auxin appeared to affect the availability of carbon sources and other phytohormones, besides cell cycle and microtubule- related proteins. Important players were also identified in each phase of AR. This is the first in depth analysis of protein changes during AR in Eucalyptus. The findings can be used in future studies to evaluate rooting competence in different genotypes and provide leads for AR improvement.
2019-08-19 | PXD013925 | Pride
Project description:Adventitious root formation at the base of plant cuttings is an innate de novo organogenesis process that allows massive vegetative propagation of many economically and ecologically important species. The early molecular events following shoot excision are not well understood. Using whole-genome microarrays, we detected significant transcriptome remodeling during 48 hours following shoot removal in Populus softwood cuttings in the absence of exogenous auxin, with 27% and 36% of the gene models showing differential abundance between 0 and 6 hours, and 6 and 24 hours, respectively. During these two time intervals, gene networks involved in protein turnover, protein phosphorylation, molecular transport and translation were among the most significantly regulated. Transgenic lines expressing a constitutively active form of the Populus type-B response regulator PtRR13 (ΔDDKPtRR13) have a delayed rooting phenotype and cause misregulation of COV1, a negative regulator of vascularization; PDR9, an auxin efflux transporter; two AP2/ERF genes with sequence similarity to TINY1. Cytokinin action appeared to disrupt root development 24 hours after shoot excision, when root founder cells are hypothesized to be sensitive to the negative effects of cytokinin. Our results suggest that PtRR13 acts downstream of cytokinin to repress adventitious root formation in intact plants, and that reduced cytokinin signaling after shoot excision enables coordinated expression of ethylene, auxin and vascularization pathways leading to adventitious root development.
2009-03-27 | GSE15049 | GEO
Project description:Toxicodendron succedaneum is an important economic tree species. In the process of establishing a regeneration system for the T. succedaneum, it was found that the axillary buds that are produced from the same tender branch after cutting are inserted into a rooting medium, with a rooting rate of 1%. When the main stem segments of the rooted tissue culture seedlings were placed on the same rooting medium, the rooting rate could reach 100%, and the complete tissue culture seedlings could be obtained. This study is based on the transcriptome and metabolome analysis, combined with the dynamic changes of endogenous hormones, investigates the key molecules, and potential mechanism in the process of adventitious root formation of TSE and TSW in T. succedaneum. The combination of transcriptomics and metabolomics revealed that the differentially expressed genes and differential metabolites in TSE and TSW were significantly enriched in plant hormone signal transduction pathway and alpha-linolenic acid metabolism pathway after 12 hours of cultivation. In the metabolome, the level of jasmonic acid in TSW is significantly lower than in TSE, and the expression levels of key genes in the JA biosynthetic pathway are also significantly lower than those in TSE. Therefore, it can be preliminarily inferred that the reduction in jasmonic acid content in TSW may be an important reason for its rooting. Furthermore, the JA content in TSE was consistently higher than it in TSW at 0,6,12 and 24 hours. Examining the dynamics of hormonal changes, When the jasmonic acid content reaches a certain concentration, it can inhibit the synthesis of auxins, thereby suppressing the formation of adventitious roots. Exogenous application of jasmonic acid and diethofencarb revealed that both high and low concentrations of JA suppress the root growth of T. succedaneum. The impact of diethofencarb treatment on the rooting of T. succedaneum is that high concentrations suppress it while low concentrations promote it. The molecular mechanism of jasmonic acid on AR formation in the T. succedaneum revealed in this study, provides a more comprehensive understanding of the AR formation mechanism in the T. succedaneum and other plants.
2025-10-04 | GSE281546 | GEO
Project description:Oligoarray analysis was used to identify genes differentially expressed in 2 mm sections of the basal stem region of wild type T89, PtAIL1 over-expressing or PtAIL1 RNAi lines of P. tremula x Populus tremuloides 24h and 72h after adventitious rooting induction.
2012-11-01 | GSE34162 | GEO