Single-cell RNA sequencing of E18.5 mouse hindstomach epithelium and bulk RNA sequencing of its derived 3D organoids across distinct YAP activity states [scRNA-Seq]

ABSTRACT: Hippo signaling plays a crucial role in the development of multiple organs. This study consists of two transcriptomic datasets.Single-cell RNA sequencing was conducted on E18.5 mouse hindstomach epithelial cells of littermate control, YAP5SA-overexpressing and Yap knockout mice, where YAP5SA represents a constitutively active form of Yap. Bulk RNA sequencing was performed to analyze transcriptional changes in 3D organoids derived from the glandular embryonic hindstomach epithelium of control and YAP5SA-overexpressing mice. Among these, organoids were cultured under two conditions: growth medium for 5 days, or growth medium for 5 days followed by differentiation medium for an additional 7 days. Comparative gene expression profiling provides valuable insights into the role of the Hippo signaling pathway in regulating diverse biological processes during glandular stomach epithelial development.

ORGANISM(S): Mus musculus

PROVIDER: GSE318022 | GEO | 2026/05/28

REPOSITORIES: GEO